Structure of the joint region and the termini of the DNA of herpes simplex virus type 1
Structure of the joint region and the termini of the DNA of herpes simplex virus type 1
复制标题
1型单纯疱疹病毒DNA连接区和末端的结构
作者：
M. Wagner;W. Summers
对1型单纯疱疹病毒Kos株DNA连接区反向重复序列中的限制性内切酶切割位点的分析表明，存在两种类型的序列异质性。第一个是280个碱基对或280个碱基对的倍数的插入，这在每个斑块纯化的病毒库中大约一半的DNA分子中发现。这些插入似乎是存在于连接处的序列的串联复制，并且与倒置的末端冗余度密切对应。第二种类型的异质性是由于在一些斑块纯化的病毒库中存在变量插入和缺失，但不是全部。将来自关节区域的限制性片段与来自末端的片段进行比较，结果表明，在观察到的最简单的单纯疱疹病毒1型DNA分子中，关节处只存在一个倒置的末端冗余副本。给出了关节区域的限制性内切酶切割位点图。
Analysis of restriction endonuclease cleavage sites within the inverted, repeated sequences in the joint region of the DNA of herpes simplex virus type 1 strain KOS revealed the presence of two types of sequence heterogeneity. The first was an insertion of 280 base pairs or multiples of 280 base pairs which was found in approximately half of all DNA molecules from every plaque-purified stock of virus. These insertions seemed to be tandem duplications of sequences which were present at the joint and correspond closely to the inverted terminal redunancy. The second type of heterogeneity was due to variable insertions and deletions which were present in some, but not all, plaque-purified virus stocks. Comparison of restriction fragments from the joint region with fragments from the termini indicated that in the simplest observed molecules of herpes simplex virus type 1 DNA, only one copy of the inverted terminal redundancy was present at the joint. A map of restriction endonuclease cleavage sites in the joint region is presented.