Impact of anesthesia on cardiac function during echocardiography in mice
Impact of anesthesia on cardiac function during echocardiography in mice
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DOI：
10.1152/ajpheart.00845.2001
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发表时间：
2002-06-01
影响因子：
4.8
通讯作者：
Ross, J
中科院分区：
文献类型：
--
作者：
Roth, DM;Swaney, JS;Ross, J
麻醉剂提供镇静和不动性，促进小鼠的超声心动图，但影响心脏功能。我们研究了腹腔内和吸入麻醉剂对超声心动图测量的影响。用腹膜内三溴乙醇（TBE）、氯胺酮-咪达唑仑（K/M）、氯胺酮-甲苯噻嗪（K/X）或吸入异氟烷（Isf）麻醉小鼠，并在5、10、15和20分钟时评估超声心动图参数。Isf产生高的初始心率（HR），在15-20分钟时降至与TBE相当的水平（类似于450次/分）和最稳定的缩短分数（%FS）和舒张末期内径（EDD）。对于TBE，%FS最初较低，但在15分钟时增加到与Isf相当（类似于45%）。K/M产生了类似的时间趋势，但所有参数的绝对值均低于TBE。K/X引起心脏抑制，表现为HR和%FS降低，EDD增加。在12天的重复研究中，Isf的重现性最好。与C57 BL/6 N小鼠相比，在C57 BL/6 J中，K/M产生更高的HR，%FS和TBE产生更小的EDD。总之，麻醉剂，超声心动图测量的时间和遗传背景都是小鼠超声心动图过程中的关键变量。
Anesthetics provide sedation and immobility facilitating echocardiography in mice, but influence cardiac function. We studied the effects of intraperitoneal and inhaled anesthetic agents on echocardiographic measurements. Mice were anesthetized with intraperitoneal tribromoethanol (TBE), ketamine-midazolam (K/M), ketamine-xylazine (K/X), or inhaled isoflurane (Isf), and echocardiographic parameters were assessed at 5, 10, 15, and 20 min. In C57BL/6N mice, Isf produced high initial heart rates (HR) that decreased to levels comparable to TBE at 15-20 min (similar to450 beats/min) and the most stable percent fractional shortening (%FS) and end-diastolic dimension (EDD). With TBE, %FS initially was low, but increased comparable to Isf (similar to45%) at 15 min. K/M produced similar time trends but lower absolute values compared with TBE for all parameters. K/X produced cardiac depression evidenced by low HR and %FS, and increased EDD. Isf was the most reproducible in repeat studies at 12 days. In C57BL/6J compared with C57BL/6N mice, K/M produced higher HR, and %FS and TBE produced smaller EDD. In conclusion, anesthetic agent, timing of echocardiographic measurements, and genetic background are all critical variables during echocardiography in mice.