Metabolism of low-density lipoprotein free cholesterol by human plasma lecithin-cholesterol acyltransferase.
Metabolism of low-density lipoprotein free cholesterol by human plasma lecithin-cholesterol acyltransferase.
复制标题
人血浆卵磷脂胆固醇酰基转移酶对低密度脂蛋白游离胆固醇的代谢。
DOI：
10.1021/bi00099a009
复制
发表时间：
1991
期刊：
影响因子：
2.9
通讯作者：
Fielding,CJ
中科院分区：
文献类型：
--
作者：
Fielding,PE;Miida,T;Fielding,CJ
Revised Manuscript Received May 10, 1991 abstract: The metabolism of cholesterol derived from [3H] cholesterol-labeled low-density lipoprotein (LDL) was determined in human blood plasma. LDL-derived free cholesterol first appeared in large a-migrating HDL (HDL2) and was then transferred to small a-HDL (HDL3) for esterification. The major part of such esters was retained within HDL of increasing size in the course of lecithin-cholesterol acyltransferase (LCAT) activity; thebalance was recovered in LDL. Transfer of preformed cholesteryl esters within HDL contributed little to the labeled cholesteryl ester accumulating in HDL2. When cholesterol for esterification was derived instead from cellmembranes, a significantly smaller proportion of this cholesteryl ester was subsequently recovered in LDL. These data suggest compartmentation of cholesteryl esters within plasma that have been formed from cell membrane or LDL free cholesterol, and the role for HDL2 as a relatively unreactive sink for LCAT-derived cholesteryl esters.
