Cell-to-cell expression variability followed by signal reinforcement progressively segregates early mouse lineages.
Cell-to-cell expression variability followed by signal reinforcement progressively segregates early mouse lineages.
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DOI：
10.1038/ncb2881
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发表时间：
2014-01
影响因子：
21.3
通讯作者：
中科院分区：
文献类型：
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现在认识到，在早期哺乳动物胚胎中，细胞之间广泛的表达异质性先于谱系的出现。为了建立一个地图的多能外胚层（EPI）与原始内胚层（PrE）谱系内的内细胞团（ICM）的小鼠囊胚分离，我们的特点是个别ICM细胞的基因表达谱。66个细胞的转录组的聚类分析表明，最初它们是不可区分的。在早期的分离，谱系特异性标记物的表达表现出没有明显的相关性，并建立了层次关系，只有在晚期囊胚。Fgf 4在分析的最早阶段表现出双峰表达，在其缺失的情况下，PrE和EPI的分化停止，这表明Fgf 4驱动ICM谱系分离并且是ICM谱系分离所需的。这些数据使我们提出了一个模型，其中随机的细胞间表达异质性，然后信号增强的基础ICM谱系分离拮抗分离等效细胞。
It is now recognized that extensive expression heterogeneities among cells precede the emergence of lineages in the early mammalian embryo. To establish a map of pluripotent epiblast (EPI) versus primitive endoderm (PrE) lineage segregation within the inner cell mass (ICM) of the mouse blastocyst, we characterised the gene expression profiles of individual ICM cells. Clustering analysis of the transcriptomes of 66 cells demonstrated that initially they are non-distinguishable. Early in the segregation, lineage-specific marker expression exhibited no apparent correlation, and a hierarchical relationship was established only in the late blastocyst. Fgf4 exhibited a bimodal expression at the earliest stage analysed, and in its absence, the differentiation of PrE and EPI was halted, indicating that Fgf4 drives, and is required for, ICM lineage segregation. These data lead us to propose a model where stochastic cell-to-cell expression heterogeneity followed by signal reinforcement underlies ICM lineage segregation by antagonistically separating equivalent cells.
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DOI：
10.1073/pnas.95.9.5082
发表时间：
1998-04-28
影响因子：
11.1
作者：
Arman, E;Haffner-Krausz, R;Lonai, P
通讯作者：
Lonai, P
影响因子：
11.8
作者：
Chazaud, Claire;Yamanaka, Yojiro;Rossant, Janet
通讯作者：
Rossant, Janet
影响因子：
11.8
作者：
Guo, Guoji;Huss, Mikael;Robson, Paul
通讯作者：
Robson, Paul
影响因子：
2.7
作者：
Artus J;Piliszek A;Hadjantonakis AK
通讯作者：
Hadjantonakis AK
影响因子：
14.8
作者：
Kurimoto, Kazuki;Yabuta, Yukihiro;Saitou, Mitinori
通讯作者：
Saitou, Mitinori