Dynamics of pH-induced spectral changes in bacteriorhodopsin.
Dynamics of pH-induced spectral changes in bacteriorhodopsin.
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pH 诱导的细菌视紫红质光谱变化的动态。
DOI：
10.1016/s0006-3495(79)85241-8
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发表时间：
1979
影响因子：
3.4
通讯作者：
M. Ottolenghi
中科院分区：
文献类型：
--
作者：
S. Druckmann;A. Samuni;M. Ottolenghi
用快速混合停流技术研究了低pH条件下细菌视紫红质的光谱漂移动力学。发色团(A605)的酸性形式的产生分两个不同的步骤发生：一个快速的过程(t1/2i=21+/-4ms)之后是一个慢得多的反应(t1/2ii=6+/-2 S)。根据视黄基发色团附近酸性基团的中和来解释观察结果，双阶段动力学被归因于发色团之间的协同效应。该方法可作为研究质子在紫膜上迁移动力学的一种工具。
The kinetics of the spectral shift induced in bacteriorhodopsin by low pH are investigated by using the rapid-mixing, stopped-flow technique. The generation of the acid form of the chromophore (A605) occurs in two distinct steps: a fast process (t1/2I = 21 +/- 4 ms) is followed by a much slower reaction (t1/2II = 6 +/- 2 s). The observations are interpreted in terms of neutralization of an acid group in the neighborhood of the retinyl chromophore, the double-staged kinetics being attributed to cooperative effects between chromophores. The method may serve as a tool for studying the kinetics of proton migration across the purple membrane.