The CMRF-35 mAb recognizes a second leukocyte membrane molecule with a domain similar to the poly Ig receptor
The CMRF-35 mAb recognizes a second leukocyte membrane molecule with a domain similar to the poly Ig receptor
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DOI：
10.1093/intimm/10.7.891
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发表时间：
1998-07-01
影响因子：
4.4
通讯作者：
Hart, DNJ
中科院分区：
文献类型：
--
作者：
Green, BJ;Clark, GJ;Hart, DNJ
CMRF-35单抗识别大多数白细胞上发现的抗原，包括单核细胞、中性粒细胞、巨噬细胞、树突状细胞、淋巴细胞和骨髓细胞亚群。通过表达克隆的方法分离到一个表达CMRF-35表位的cDNA，这预示着一个属于Ig超家族的I型细胞表面糖蛋白。在这里，我们证明了CMRF-35单抗识别的表位比CMRF-35 mRNA的表达分析所建议的更广泛地分布在造血细胞和细胞系上。此外，我们还分离到一个新的cDNA(CMRF-35-H9)，它编码一个也被CMRF-35单抗识别的蛋白质产物。该基因产物为I型细胞表面糖蛋白，具有单一的Ig V样结构域。虽然这两个分子的胞外V型结构域序列非常相似，但它们的其余序列之间几乎没有相似之处。这两个转录本彼此独立表达，它们的存在解释了CMRF-35单抗结合和mRNA分析之间的差异。CMRF-35-H9的胞质尾部含有与某些白细胞表面受体中发现的抑制基序相似的基序。它们在造血细胞中的表达表明，这两个分子在白细胞功能的调节中可能发挥着不同但又相互关联的作用。
The CMRF-35 mAb recognizes an antigen found on most leukocytes including monocytes, neutrophils, macrophages, dendritic cells, and subpopulations of lymphocytes and bone marrow cells. A cDNA expressing the CMRF-35 epitope was isolated by expression cloning and this predicts for a type I cell surface glycoprotein belonging to the Ig superfamily. Here we demonstrate that the CMRF-35 mAb recognizes an epitope more widely distributed on hemopoietic cells and cell lines than suggested by expression analysis of the CMRF-35 mRNA. Furthermore, we have isolated a novel cDNA (CMRF-35-H9) that encodes a protein product also recognized by the CMRF-35 mAb. This cDNA product is a type I cell surface glycoprotein with a single Ig V-like domain. Although the sequences of the extracellular V-like domains of the two molecules are very similar, there is little similarity between the remainder of their sequences. The two transcripts are expressed independently of each other, and their presence accounts for the discrepancy between CMRF-35 mAb binding and mRNA analysis. The cytoplasmic tail of CMRF-35-H9 contains motifs similar to the inhibitory motifs found in some leukocyte surface receptors. Their expression in hemopoietic cells suggests that these two molecules may play distinct but related roles in the regulation of leukocyte function.