Identification of genes controlling growth polarity in the budding yeast Saccharomyces cerevisiae: a possible role of N-glycosylation and involvement of the exocyst complex.
Identification of genes controlling growth polarity in the budding yeast Saccharomyces cerevisiae: a possible role of N-glycosylation and involvement of the exocyst complex.
复制标题
识别出芽酵母酿酒酵母中控制生长极性的基因：N-糖基化和外囊复合体参与的可能作用。
DOI：
10.1093/genetics/147.2.421
复制
发表时间：
1997
期刊：
影响因子：
3.3
通讯作者：
Reed,SI
中科院分区：
文献类型：
--
作者：
Mondésert,G;Clarke,DJ;Reed,SI
细胞周期中分泌极性和细胞表面生长的调控是酿酒酵母正常形态发生和生存的关键。从各向同性细胞表面生长到极化生长的转变是芽萌发所必需的，分泌到芽颈的再极化是细胞分离所必需的。虽然肌动蛋白细胞骨架的改变与这些分泌极性的变化有关，但很明显，参与分泌的其他细胞系统可能是细胞周期调节的目标。为了研究细胞周期进程与肌动蛋白极化调节并行和下游的分泌极性变化耦合的机制，我们对极化生长特异性缺陷但肌动蛋白细胞骨架结构正常的突变体进行了筛选。这些突变体分为三类：部分n -糖基化缺陷的，与外囊的特定缺陷有关的，第三类糖基化没有缺陷，与外囊没有联系。这些结果提出了一种可能性，即n连接糖基化的变化可能涉及连接细胞周期进程和分泌极性的信号，并且胞囊可能在将分泌机制耦合到极化的肌动蛋白细胞骨架中具有调节功能。
The regulation of secretion polarity and cell surface growth during the cell cycle is critical for proper morphogenesis and viability ofSaccharomyces cerevisiae. A shift from isotropic cell surface growth to polarized growth is necessary for bud emergence and a repolarization of secretion to the bud neck is necessary for cell separation. Although alterations in the actin cytoskeleton have been implicated in these changes in secretion polarity, clearly other cellular systems involved in secretion are likely to be targets of cell cycle regulation. To investigate mechanisms coupling cell cycle progression to changes in secretion polarity in parallel with and downstream of regulation of actin polarization, we implemented a screen for mutants defective specifically in polarized growth but with normal actin cytoskeleton structure. These mutants fell into three classes: those partially defective inN-glycosylation, those linked to specific defects in the exocyst, and a third class neither defective in glycosylation nor linked to the exocyst. These results raise the possibility that changes inN-linked glycosylation may be involved in a signal linking cell cycle progression and secretion polarity and that the exocyst may have regulatory functions in coupling the secretory machinery to the polarized actin cytoskeleton.