PLATELET-AGGREGATION BY BASEMENT-MEMBRANE - ASSOCIATED COLLAGENS
PLATELET-AGGREGATION BY BASEMENT-MEMBRANE - ASSOCIATED COLLAGENS
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DOI：
10.1016/0049-3848(80)90333-3
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发表时间：
1980-01-01
影响因子：
7.5
通讯作者：
MACINTYRE, DE
中科院分区：
文献类型：
--
作者：
BARNES, MJ;BAILEY, AJ;MACINTYRE, DE
IV型胶原（来自牛前晶状体囊）或V型胶原（来自人胎盘或牛肺实质）的溶液即使在37度的孵养中预处理也不能聚集血小板。C或透析0.02 M-Na2HPO4在4度。C，在间质胶原的情况下，已知诱导形成高活性原纤维（具有67 nm轴向周期性）的程序。含有IV型原位胶原的完整晶状体囊也无活性。IV型或V型胶原（来自人胎盘）溶液在酸性pH条件下对ATP进行透析，产生SLS[段长间距]聚集体，这种聚集体能够像I型胶原一样诱导血小板聚集。V型胶原蛋白溶液（取自人胎盘）与生理盐水在25度下透析。C产生67nm周期的原纤维，可诱导血小板反应。从人胎盘提取的IV型胶原蛋白与0.02 M-Na2HPO4进行透析，得到活性原纤维，在这种情况下，是非条纹的。决定胶原诱导血小板聚集能力的是四级结构而不是分子组成。在适当的条件下，胶原基膜类型可以在体外表现出适合血小板活性的结构。
Solutions of type IV collagen (from bovine anterior lens capsules) or of type V (from human placenta or bovine lung parenchyma) failed to aggregate platelets even when pretreated by incubation at 37.degree. C or by dialysis against 0.02 M-Na2HPO4 at 4.degree. C, procedures known to induce the formation of highly active fibrils (with a 67 nm axial periodicity) in the case of the interstitial collagens. Intact lens capsule containing type IV collagen in situ was also inactive. Dialysis of solutions of type IV or V collagens (from human placenta) against ATP at acid pH yielded SLS [segment long spacing] aggregates that were able, like those of type I collagen, to induce platelet aggregation. Dialysis of a solution of type V collagen (from human placenta) against physiological saline at 25.degree. C yielded fibrils at 67 nm periodicity that could induce a platelet response. Dialysis of type IV collagen from human placenta against 0.02 M-Na2HPO4 yielded active fibrils, in this case, non-striated. Quaternary structure rather than molecular composition determines the ability of collagen to induce platelet aggregation. The basement membrane-types of collagen can, under appropriate conditions in vitro, exhibit a configuration suitable for platelet activity.