Production of colony-stimulating factor by malignant leukocytes.
Production of colony-stimulating factor by malignant leukocytes.
复制标题
恶性白细胞产生集落刺激因子。
作者：
D. Golde;B. Rothman;M. Cline
有相当多的证据支持集落刺激因子作为白细胞生成的体液调节因子的作用。白血病患者血清和尿液中的脑脊液水平以及白血病细胞对脑脊液的体外反应性的数据表明，白血病是一种原发的白细胞调节障碍。我们研究了脑脊液中白血病细胞的产生问题。用两层琼脂集落试验技术检测培养的白血病细胞条件培养液对正常人骨髓的集落刺激活性。急性髓系白血病和慢性粒细胞白血病急变期患者的细胞不表达脑脊液，急性淋巴细胞白血病细胞也不表达。慢性粒细胞白血病慢性期患者和两名急性髓-单核细胞白血病患者的细胞产生脑脊液。急性白血病患者脑脊液的细胞产生与单核-巨噬细胞系的形态和功能成熟密切相关。有证据表明，白血病人群中的贴壁细胞主要负责脑脊液的产生。我们解释这些数据是为了表明肿瘤造血细胞可能产生与其单核白细胞分化能力有关的脑脊液。
There is considerable evidence supporting a role for colony-stimulating factor (CSF) as a humoral regulator of leukopoiesis. Data on CSF levels in the serum and urine of patients with leukemia and on the in vitro responsiveness of leukemic cells to CSF have suggested a basis for considering leukemia as a primary disorder of leukopoietic regulation. We examined the question of leukemic cell production of CSF. Conditioned medium from cultured leukemic cells was tested for colony-stimulating activity against normal human bone marrow using a two-layer agar colony assay technique. The cells from patients with acute myelogenous leukemia and a patient with chronic myelogenous leukemia in blast crisis did not elaborate CSF nor did acute lymphocytic leukemia cells. CSF production was documented with cells obtained from patients with chronic myelogenous leukemia in the chronic phase and two patients with acute myelomonocytic leukemia. In acute leukemia the cellular production of CSF correlated closely with morphologic and functional maturation along the monocyte-macrophage line. Evidence was obtained that the adherent cells within the leukemic population were primarily responsible for CSF production. We interpret these data to indicate that neoplastic hematopoietic cells may produce CSF in relation to their capacity for mononuclear leukocyte differentiation.