Histochemical and cytochemical localization of (Na+-K+)-activated adenosine triphosphatase in the acini of dog submandibular glands.
Histochemical and cytochemical localization of (Na+-K+)-activated adenosine triphosphatase in the acini of dog submandibular glands.
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(Na -K ) 激活的腺苷三磷酸酶在狗颌下腺腺泡中的组织化学和细胞化学定位。
DOI：
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发表时间：
1978
影响因子：
3.2
通讯作者：
Y. Imai
中科院分区：
文献类型：
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作者：
I. Nakagaki;T. Goto;S. Sasaki;Y. Imai
光镜下观察了（Na+-K+）活化的腺苷三磷酸酶（Na+-K+）对犬颌下腺腺泡细胞对硝基苯磷酸酶（p-NPPase）的活性。新鲜冷冻切片的p-NPPase反应产物定位于腺泡菌基部，在含10(-3)Mouabain培养基或无k培养基中孵育后，反应产物消失。电镜下发现，atp酶的反应产物均位于浆液细胞和黏液细胞的基底外侧质膜上。在腺泡细胞腔质膜的微绒毛上也有少量的反应产物。这种atp酶反应产物在浆液和黏液细胞上的定位似乎与光镜下在腺泡细胞上观察到的p-NPPase活性相吻合。对上述酶的分布与质膜阳离子运输的关系给出了可能的解释。本文还讨论了犬颌下腺腺泡细胞的结构和功能不对称特性。
p-Nitrophenyl phosphatase (p-NPPase) activity of (Na+-K+)-activated adenosine triphosphatase ((Na+-K+)-ATPase) on the acinar cells of dog submandibular gland was demonstrated by using light microscopy. The reaction products of p-NPPase of fresh frozen sections were seen to be localized on the basal parts of acini, and disappeared when the sections were incubated in medium containing 10(-3) Mouabain or in a K-free medium. Under the electron microscope, the reaction products of ATPase were found to be localized on the basolateral plasma membrane of both serous and mucous cells. On the microvilli of the luminal plasma membrane of the acinar cell, a small quantity of the reaction products was also present. This localization of ATPase reaction products on the serous and mucous cells seemed to coincide well with that of p-NPPase activity observed on the acini under light microscopy. Possible explanations are given regarding distribution of the above mentioned enzymes in relation to the cation transport of the plasma membrane. Structural and functional asymmetrical properties of acinar cells of the dog submandibular gland are also discussed.