Self-Defensive Biomaterial Coating Against Bacteria and Yeasts: Polysaccharide Multilayer Film with Embedded Antimicrobial Peptide
Self-Defensive Biomaterial Coating Against Bacteria and Yeasts: Polysaccharide Multilayer Film with Embedded Antimicrobial Peptide
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DOI：
10.1002/adfm.201300416
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发表时间：
2013-10-11
影响因子：
19
通讯作者：
Boulmedais, F.
中科院分区：
文献类型：
--
作者：
Cado, G.;Aslam, R.;Boulmedais, F.
预防医疗植入物的病原体定殖是一个主要的医疗和财务问题，因为微生物感染构成手术或重症监护后最严重的并发症之一。将抗菌分子固定在生物材料表面是防止生物被膜形成的有效方法。在本文中，报道了针对细菌和酵母的第一种自我防御涂层，其中抗微生物肽的释放是由病原体本身引起的膜的酶促降解引发的。基于生物相容性和可生物降解的多糖多层膜功能化的透明质酸由cateslytin（CTL），一种内源性宿主防御性抗菌肽，和壳聚糖（HA-CTL-C/CHI）沉积在一个平面表面上的抗菌和抗真菌涂层的目的。孵育24小时后，HA-CTL-C/CHI膜完全抑制革兰氏阳性金黄色葡萄球菌细菌和白色念珠菌酵母菌的发展，这些细菌和酵母菌是护理相关疾病中遇到的常见和毒性病原体。由病原体分泌的羟乙稀酸酶导致膜降解和肽的抗微生物作用。此外，在HA-CTL-C/CHI膜上有限的成纤维细胞粘附，而没有细胞毒性，突出了预防导管或气管导管上的感染的医学相关应用，其中纤维组织包封是不期望的。
Prevention of pathogen colonization of medical implants is a major medical and financial issue since infection by microorganisms constitutes one of the most serious complications after surgery or critical care. Immobilization of antimicrobial molecules on biomaterials surfaces is an efficient approach to prevent biofilm formation. Herein, the first self-defensive coating against both bacteria and yeasts is reported, where the release of the antimicrobial peptide is triggered by enzymatic degradation of the film due to the pathogens themselves. Biocompatible and biodegradable polysaccharide multilayer films based on functionalized hyaluronic acid by cateslytin (CTL), an endogenous host-defensive antimicrobial peptide, and chitosan (HA-CTL-C/CHI) are deposited on a planar surface with the aim of designing both antibacterial and antifungal coating. After 24 h of incubation, HA-CTL-C/CHI films fully inhibit the development of Gram-positive Staphylococcus aureus bacteria and Candida albicans yeasts, which are common and virulent pathogens agents encountered in care-associated diseases. Hyaluronidase, secreted by the pathogens, leads to the film degradation and the antimicrobial action of the peptide. Furthermore, the limited fibroblasts adhesion, without cytotoxicity, on HA-CTL-C/CHI films highlights a medically relevant application to prevent infections on catheters or tracheal tubes where fibrous tissue encapsulation is undesirable.