Molecular characterization of a wheat-Psathyrostachys huashanica Keng 2Ns disomic addition line with resistance to stripe rust
Molecular characterization of a wheat-Psathyrostachys huashanica Keng 2Ns disomic addition line with resistance to stripe rust
复制标题
DOI：
10.1007/s00438-014-0844-2
复制
发表时间：
2014-10-01
影响因子：
3.1
通讯作者：
Chen, Xinhong
中科院分区：
文献类型：
--
作者：
Du, Wanli;Wang, Jing;Chen, Xinhong
通过基因组原位杂交、分子标记分析和农艺性状评价，对华山新麦草衍生品系3-6-4-1进行了鉴定。GISH分析表明，3-6-4-1含有42条小麦染色体和1对华山杨染色体。利用亲本和附加系中7个小麦同源群体的EST-STS多基因座标记，对引进的华山杨染色体进行了同源关系分析。位于小麦同源第2组染色体上的12个EST-STS标记在3-6-4-1处扩增出多态带，为华山松所特有。利用染色体特异标记鉴定了一对导入的NS染色体对，该染色体对属于同源群2。苗期和成株期分别接种3-6-4-1和3-6-4-1的3-6-4-1和3-6-4-1对条锈病的抗性，这可能与其亲本华山杨有关。我们还比较了一整套小麦-P。对华山二体附加系(1NS-7 NS，2n=44=22II)的农艺性状和形态性状进行了评价，结果表明，3-6-4-1的穗部性状较其亲本有所改善。3-6-4-1中的华山杨2 NS染色体特异分子标记可用于抗条锈病育种的分子标记辅助选择。该品系可作为供体来源，将华山松新的优良基因导入小麦，扩大其遗传多样性，为小麦育种提供新的种质资源。
We characterized a wheat-Psathyrostachys huashanica derived line 3-6-4-1 based on genomic in situ hybridization (GISH), molecular marker analysis, and agronomic trait evaluations. The GISH investigations showed that the 3-6-4-1 contained 42 wheat chromosomes and a pair of P. huashanica chromosomes. The homoeologous relationships of the introduced P. huashanica chromosomes were determined using EST-STS multiple loci markers from seven wheat homoeologous groups in the parents and the addition line. Twelve EST-STS markers located on the homoeologous group 2 chromosomes of wheat amplified polymorphic bands in 3-6-4-1, which were unique to P. huashanica. An introduced Ns chromosome pair that belonged to homoeologous group 2 was identified using chromosome-specific markers. Inoculation with isolates of the stripe rust pathotypes, CYR31, CYR32, and SY11-14, and mixed races (CYR31, CYR32, and SY11-14) in the seeding and adult stage, respectively, showed that 3-6-4-1 was generally resistant to stripe rust, which was probably attributable to its P. huashanica parent. We also compared a complete set of wheat-P. huashanica disomic addition lines (1Ns-7Ns, 2n = 44 = 22II) to assess their agronomic traits and morphological characteristics, which showed that 3-6-4-1 had improved spike traits compared with its parents. The P. huashanica 2Ns chromosome-specific molecular markers in 3-6-4-1 could be useful for marker-assisted selection in breeding programs to combat stripe rust. This line can be used as a donor source to introduce novel excellent genes from P. huashanica into wheat to widen its genetic diversity, thereby providing new germplasms for wheat breeding.