Effect of water temperature and fish biomass on environmental DNA shedding, degradation, and size distribution
Effect of water temperature and fish biomass on environmental DNA shedding, degradation, and size distribution
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DOI：
10.1002/ece3.4802
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发表时间：
2019-02-01
影响因子：
2.6
通讯作者：
Minamoto, Toshifumi
中科院分区：
文献类型：
--
作者：
Jo, Toshiaki;Murakami, Hiroaki;Minamoto, Toshifumi
环境DNA （eDNA）分析已经成功地检测了各种水生环境中的生物。然而，关于eDNA的基本信息很少，包括eDNA脱落和降解过程。研究结果表明，eDNA的脱落、降解和大小分布随水温和鱼类生物量的变化而变化。鱼缸实验包括4个温度水平和3个鱼生物量水平。对日本青花鱼（Trachurus japonicus）取鱼前后的总eDNA和大小分型eDNA进行定量分析。结果表明，水温越高、鱼类生物量越大，eDNA脱落率越高；温度越高、鱼类生物量越大，eDNA衰变率也越高。此外，在较高的温度下，小尺寸eDNA组分的比例较大，并且这些比例在鱼类生物量中有所不同。将鱼从水族箱中取出后，当eDNA大小分数为10 μ m时，eDNA的百分比暂时下降，而较小的分数则增加。这些结果有可能使eDNA分析在未来得到更广泛的应用。
Environmental DNA (eDNA) analysis has successfully detected organisms in various aquatic environments. However, there is little basic information on eDNA, including the eDNA shedding and degradation processes. This study focused on water temperature and fish biomass and showed that eDNA shedding, degradation, and size distribution varied depending on water temperature and fish biomass. The tank experiments consisted of four temperature levels and three fish biomass levels. The total eDNA and size-fractioned eDNA from Japanese Jack Mackerels (Trachurus japonicus) were quantified before and after removing the fish. The results showed that the eDNA shedding rate increased at higher water temperature and larger fish biomass, and the eDNA decay rate also increased at higher temperature and fish biomass. In addition, the small-sized eDNA fractions were proportionally larger at higher temperatures, and these proportions varied among fish biomass. After removing the fish from the tanks, the percentage of eDNA temporally decreased when the eDNA size fraction was >10 mu m, while the smaller size fractions increased. These results have the potential to make the use of eDNA analysis more widespread in the future.