Constitutive or seed-specific overexpression of Arabidopsis G-protein γ subunit 3 (AGG3) results in increased seed and oil production and improved stress tolerance in Camelina sativa
Constitutive or seed-specific overexpression of Arabidopsis G-protein γ subunit 3 (AGG3) results in increased seed and oil production and improved stress tolerance in Camelina sativa
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DOI：
10.1111/pbi.12115
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发表时间：
2014-01-01
影响因子：
13.8
通讯作者：
Pandey, Sona
中科院分区：
文献类型：
--
作者：
Choudhury, Swarup Roy;Riesselman, Adam J.;Pandey, Sona
由 G、G 和 G 亚基组成的异三聚体 G 蛋白在介导植物中的多种信号传导途径中发挥着不可或缺的作用。最近发现的一种新的植物特异性 G 蛋白 AGG3 被认为是拟南芥中器官大小的重要调节剂和应激反应的调节剂，而其在水稻中的潜在同源物是种子大小和穗分枝的主要数量性状基因座。为了评估 AGG3 对种子和油料产量提高的作用，该基因在十字花科油籽作物 Camelina sativa 中过表达。对多个纯合 T4 转基因亚麻荠品系的分析表明，AGG3 的组成型过表达导致更快的营养生长和生殖生长，并伴随着光合效率的提高。此外，当在种子组织中组成型或特异性表达时，AGG3被发现可使每株植物的种子大小、种子质量和种子数量增加15%-40%，有效地导致每株植物的油产量显着提高。过表达 AGG3 的亚麻荠植物也表现出改善的胁迫耐受性。这些观察结果表明 AGG3 在调节两个关键产量参数（种子性状和植物胁迫反应）中的作用之间存在密切联系，并揭示了一种显着提高农作物产量的有效生物技术工具。
Heterotrimeric G-proteins consisting of G, G and G subunits play an integral role in mediating multiple signalling pathways in plants. A novel, recently identified plant-specific G protein, AGG3, has been proposed to be an important regulator of organ size and mediator of stress responses in Arabidopsis, whereas its potential homologs in rice are major quantitative trait loci for seed size and panicle branching. To evaluate the role of AGG3 towards seed and oil yield improvement, the gene was overexpressed in Camelina sativa, an oilseed crop of the Brassicaceae family. Analysis of multiple homozygous T4 transgenic Camelina lines showed that constitutive overexpression of AGG3 resulted in faster vegetative as well as reproductive growth accompanied by an increase in photosynthetic efficiency. Moreover, when expressed constitutively or specifically in seed tissue, AGG3 was found to increase seed size, seed mass and seed number per plant by 15%-40%, effectively resulting in significantly higher oil yield per plant. AGG3 overexpressing Camelina plants also exhibited improved stress tolerance. These observations draw a strong link between the roles of AGG3 in regulating two critical yield parameters, seed traits and plant stress responses, and reveal an effective biotechnological tool to dramatically increase yield in agricultural crops.