SmSak, the second Polo-like kinase of the helminth parasite Schistosoma mansoni: conserved and unexpected roles in meiosis.
SmSak, the second Polo-like kinase of the helminth parasite Schistosoma mansoni: conserved and unexpected roles in meiosis.
复制标题
DOI：
10.1371/journal.pone.0040045
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Dissous C
中科院分区：
文献类型：
--
作者：
Long T;Vanderstraete M;Cailliau K;Morel M;Lescuyer A;Gouignard N;Grevelding CG;Browaeys E;Dissous C
Polo样激酶（Plks）是一个保守的调节因子家族，在整个细胞周期中的各种事件，从酵母中的一个Plk扩展到哺乳动物中的五个Plk（Plk 1 -5）。Plk 1是Plk家族中最具特征的成员，与果蝇的创始成员波罗同源，并且通过触发G2/M转换在细胞周期进展中起主要作用。Plk 4/Sak（Snk（血清诱导激酶）akin激酶）是该家族的一个独特成员，在结构上不同于其他Plk成员，在中心粒复制中具有重要功能。曼氏血吸虫（Schistosoma mansoni）的基因组仅包含两个Plk基因，分别编码SmPlk 1和SmSak。SmPlk 1已被证明是配子发生和寄生虫繁殖所必需的。在这项工作中，原位杂交表明，结构保守的Plk 4蛋白，SmSak，主要表达在雌虫卵巢和卵黄。SmSak在非洲爪蟾卵母细胞中的表达证实了其Plk 4在中心粒扩增中的保守功能。此外，SmSak在G2阻断的非洲爪蟾卵母细胞减数分裂进程中的功能分析表明，与SmPlk 1相反，SmSak在没有内源性Plk 1（Plx 1）的情况下不能诱导G2/M转换。出乎意料的是，减数分裂进程自发观察Plx 1耗尽的卵母细胞共表达SmSak和SmPlk 1。SmSak和SmPlk 1之间的分子相互作用通过两种蛋白质的共免疫沉淀来证实。这些数据表明，Plk 1和Plk 4蛋白有可能在细胞中相互作用和交叉激活，从而首次归因于Plk 4蛋白在减数分裂/有丝分裂进入中的潜在作用。SmSak在减数分裂中的这种意想不到的作用可能与进一步考虑这种新的Plk在染色体生殖中的功能有关。
Polo-like kinases (Plks) are a family of conserved regulators of a variety of events throughout the cell cycle, expanded from one Plk in yeast to five Plks in mammals (Plk1-5). Plk1 is the best characterized member of the Plk family, homolog to the founding member Polo of Drosophila, and plays a major role in cell cycle progression by triggering G2/M transition. Plk4/Sak (for Snk (Serum-inducible kinase) akin kinase) is a unique member of the family, structurally distinct from other Plk members, with essential functions in centriole duplication. The genome of the trematode parasite Schistosoma mansoni contains only two Plk genes encoding SmPlk1 and SmSak. SmPlk1 has been shown already to be required for gametogenesis and parasite reproduction. In this work, in situ hybridization indicated that the structurally conserved Plk4 protein, SmSak, was largely expressed in schistosome female ovary and vitellarium. Expression of SmSak in Xenopus oocytes confirmed its Plk4 conserved function in centriole amplification. Moreover, analysis of the function of SmSak in meiosis progression of G2-blocked Xenopus oocytes indicated that, in contrast to SmPlk1, SmSak cannot induce G2/M transition in the absence of endogenous Plk1 (Plx1). Unexpectedly, meiosis progression was spontaneously observed in Plx1-depleted oocytes co-expressing SmSak and SmPlk1. Molecular interaction between SmSak and SmPlk1 was confirmed by co-immunoprecipitation of both proteins. These data indicate that Plk1 and Plk4 proteins have the potential to interact and cross-activate in cells, thus attributing for the first time a potential role of Plk4 proteins in meiosis/mitosis entry. This unexpected role of SmSak in meiosis could be relevant to further consider the function of this novel Plk in schistosome reproduction.
登录
查看更多内容
DOI：
10.1590/s0001-37652011000200022
发表时间：
2011-06-01
期刊：
Anais da Academia Brasileira de Ciências
影响因子：
--
作者：
Dissous, Colette;Grevelding, Christoph G;Long, Thavy
通讯作者：
Long, Thavy
影响因子：
6.7
作者：
Beckmann S;Buro C;Dissous C;Hirzmann J;Grevelding CG
通讯作者：
Grevelding CG
影响因子：
14.9
作者：
Andrysik Z;Bernstein WZ;Deng L;Myer DL;Li YQ;Tischfield JA;Stambrook PJ;Bahassi el M
通讯作者：
Bahassi el M
影响因子：
9.2
作者：
Eckerdt, Frank;Yamamoto, Tomomi M.;Maller, James L.
通讯作者：
Maller, James L.
影响因子：
21.3
作者：
Habedanck, R;Stierhof, YD;Nigg, EA
通讯作者：
Nigg, EA