Soluble factors secreted by macrophage-like cells in vitro cause osteoprogenitor cell detachment
Soluble factors secreted by macrophage-like cells in vitro cause osteoprogenitor cell detachment
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DOI：
10.1007/s002239900564
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发表时间：
1998-12-01
影响因子：
4.2
通讯作者：
Jones, S
中科院分区：
文献类型：
--
作者：
Evans, CE;Jones, S
从各种骨骼部位获得成年兔骨的外植体培养物，目的是将细胞用于新型骨移植物中。外植体培养物含有大量的酯酶阳性，碱性磷酸酶阴性，TRAP阴性的巨噬细胞样细胞（MP），以及成骨细胞样骨祖细胞（OP）细胞（碱性磷酸酶阳性）。通过细胞计数和Ma测定评估OP数量。MP细胞的存在似乎通过细胞分离和细胞裂解引起培养物中OP数量的减少。此外，传代后，许多OP在MP存在下无法重新附着到培养容器上，这取决于可用于重新附着的表面积。通过特异性酶联免疫吸附试验（ELISA）证明这些细胞培养物的培养基中存在肿瘤坏死因子α（TNF α）。在共培养物中，MP数量和TNF α浓度之间存在直接关系，MP数量和OP数量之间存在反比关系。当OP细胞暴露于不同浓度的rTNF α代替MP时也发现了这一点。用抗TNF α孵育。这些结果表明，MP能够引起OP细胞的脱离和溶解，可能是通过在体外分泌可溶性因子，可能是TNF α。
Explant cultures of adult rabbit bone were obtained from a variety of skeletal sites, with the intention of using the cells in a new type of bone graft. The explant cultures contained large numbers of esterase-positive, alkaline phosphatase-negative, TRAP-negative macrophagelike cells (MP), as well as osteoblast-like osteoprogenitor (OP) cells (alkaline phosphatase-positive). OP numbers were assessed by cell counts and Ma assay. The presence of the MP cells appeared to give rise to a reduction in OP numbers in culture, through cell detachment and cell lysis. In addition, after passaging, many OP were unable to reattach to the culture vessels in the presence of MP, depending upon the surface area available for reattachment. The presence of tumor necrosis factor alpha (TNF alpha) in culture medium from these cell cultures was demonstrated by a specific enzyme-linked immunosorbent assay (ELISA). A direct relationship was demonstrated between MP numbers and TNF alpha concentration and an inverse relationship between MP numbers and OP numbers in co-cultures. This was also found when OP cells were exposed to different concentrations of rTNF alpha, in place of the MP. Incubation with anti-TNF alpha. inhibited the effect of MP and TNF alpha on OP. These results suggest that MP are able to cause detachment and lysis of OP cells, probably by secretion of a soluble factor in vitro which may be TNF alpha.