Basement membrane and connective tissue proteins in early lesions of Kaposi's sarcoma associated with AIDS.
Basement membrane and connective tissue proteins in early lesions of Kaposi's sarcoma associated with AIDS.
复制标题
与艾滋病相关的卡波西肉瘤早期病变中的基底膜和结缔组织蛋白。
DOI：
10.1111/1523-1747.ep12273506
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Greenspan,JS
中科院分区：
文献类型：
--
作者：
Kramer,RH;Fuh,GM;Hwang,CB;Conant,MA;Greenspan,JS
近三分之一被诊断为获得性免疫缺陷综合征（艾滋病）的年轻同性恋男子发展为皮肤卡波西肉瘤（KS）的扩散形式。虽然KS细胞的组织发生尚不清楚，但有证据表明这些异常细胞是内皮细胞。我们已经研究了存在和分布的结缔组织特异性和基底膜特异性的大分子通过间接免疫荧光和免疫过氧化物酶染色的冷冻切片在早期皮肤病变的KS从个人艾滋病。KS细胞通常排列在网状真皮的胶原束之间的空间中。当结缔组织特异性糖蛋白纤连蛋白染色时，所有卡波西肉瘤病变显示出强烈的染色模式，揭示了一系列复杂的线性抗原沉积物，勾勒出胶原束的外表面。层粘连蛋白和IV型胶原蛋白（两者均为基底膜特异性大分子）的抗体产生与抗纤连蛋白抗血清相似的强烈染色模式，表明所有3种抗原紧密共分布。与此相反，抗体的I型胶原蛋白，真皮的主要胶原蛋白，均匀染色的KS病变和正常对照皮肤中的胶原束。因子VIII相关抗原（一种血管内皮特异性抗原）的抗血清经常染色KS病变，但染色模式是弥漫性的，强度可变。结果提示KS细胞来源于微血管内皮细胞，并保持其分泌基底膜特异性大分子的分泌表型。
Nearly one-third of all young homosexual men diagnosed as having acquired immune-deficiency syndrome (AIDS) develop a disseminated form of dermal Kaposi's sarcoma (KS). Although the histogenesis of KS cells is unclear, certain evidence suggests that the aberrant cells are of endothelial derivation. We have examined the presence and distribution of connective tissue-specific and basement membrane-specific macromolecules by indirect immunofluorescence and immunoperoxidase staining of frozen sections in early cutaneous lesions of KS from individuals with AIDS. The KS cells typically line the spaces between collagen bundles of the reticular dermis. When stained for the connective tissue-specific glycoprotein fibronectin, all Kaposi's sarcoma lesions showed an intense staining pattern, revealing a complex array of linear deposits of antigen that outlined the exterior surface of the collagen bundles. Antibodies to laminin and type IV collagen, both basement membrane- specific macromolecules, produced an intense staining pattern similar to that found with the anti-fibronectin antiserum, indicating that all 3 antigens are closely codistributed. In contrast, antibodies to type I collagen, the major collagen of the dermis, uniformly stained the collagen bundles in the KS lesions and in the normal control skin. Antiserum to factor VIII-associated antigen, an antigen specific to blood vascular endothelium, frequently stained the KS lesions but the staining pattern was diffuse and of variable intensity. The results suggest that KS cells are derived from the endothelium of the blood microvasculature and maintain their secretory phenotype of secreting basement membrane-specific macromolecules.