Effects of aqueous extract of Casearia sylvestris (Flacourtiaceae) on actions of snake and bee venoms and on activity of phospholipases A2
Effects of aqueous extract of Casearia sylvestris (Flacourtiaceae) on actions of snake and bee venoms and on activity of phospholipases A2
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DOI：
10.1016/s0305-0491(00)00237-6
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发表时间：
2000-09-01
影响因子：
2.2
通讯作者：
Homsi-Brandeburgo, MI
中科院分区：
文献类型：
--
作者：
Borges, MH;Soares, AM;Homsi-Brandeburgo, MI
测定了产于巴西牧场的木麻黄叶粗提物对蜜蜂和几种蛇毒的磷脂酶A(2)活性和部分生物活性的抑制作用，以及对几种分离的磷脂酶A(2)S的抑制作用。对含I、II、III类磷脂酶A(2)S的蛇毒有部分抑制作用，对纯化的蛇毒中对II类磷脂酶S的抑制作用最强，对I类磷脂酶(2)伪糊精的抑制作用相对较弱。此外，小叶锦鸡儿提取物对4种牛膝蛇毒粗毒和9种纯化的中国人民解放军(2)S(包括Lys-49和Asp-49变异体)的肌毒活性有明显的抑制作用。除伪糊精外，该提取物均能抑制几种分离的解放军(2)S的抗凝血活性。此外，它还能部分降低Moojeni和B.jaracussu蛇毒以及肌毒素MjTX-II和BthTX-I的致肿活性。该提取物还可延长注射几种蛇毒致死剂量的小鼠的存活时间，并中和几种纯化的解放军(2)肌毒素的致死作用。研究结果表明，马尾藻中含有丰富的磷脂酶A(2)抑制剂。(C)2000 Elsevier Science Inc.保留所有权利。
The crude aqueous extract from the leaves of Casearia sylvestris, a plant found in Brazilian open pastures, was assayed for its ability to inhibit phospholipase A(2) (PLA(2)) activity and some biological activities of bee and several snake venoms, and of a number of isolated PLA(2)s. The extract induced partial inhibition of the PLA(2) activity of venoms containing class I, II and III PLA(2)s. When tested against the purified toxins, it showed the highest efficacy against class II PLA(2)s from viperid venoms, being relatively ineffective against the class I PLA(2) pseudexin. In addition, C. sylvestris extract significantly inhibited the myotoxic activity of four Bothrops crude venoms and nine purified myotoxic PLA(2)s, including Lys-49 and Asp-49 variants. The extract was able to inhibit the anticoagulant activity of several isolated PLA(2)s, with the exception of pseudexin. Moreover, it partially reduced the edema-inducing activity of B. moojeni and B. jararacussu venoms, as well as of myotoxins MjTX-II and BthTX-I. The extract also prolonged the survival time of mice injected with lethal doses of several snake venoms and neutralized the lethal effect induced by several purified PLA(2) myotoxins. It is concluded that C. sylvestris constitutes a rich source of PLA(2) inhibitors. (C) 2000 Elsevier Science Inc. All rights reserved.