Comparative transcriptome analysis of salt-tolerant wheat germplasm lines using wheat genome arrays
Comparative transcriptome analysis of salt-tolerant wheat germplasm lines using wheat genome arrays
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DOI：
10.1016/j.plantsci.2007.06.005
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发表时间：
2007-09-01
期刊：
影响因子：
5.2
通讯作者：
Wang, Richard R. -C.
中科院分区：
文献类型：
--
作者：
Mott, Ivan W.;Wang, Richard R. -C.
耐盐小麦品系W 4909和W 4910是由AJDAJ 5（带有一对来自Thinopyrum junceum的Eb染色体的二体附加系）和Ph'（具有来自斯卑尔脱山羊草（Aegilops speltoides）的Ph'等位基因的品系，该等位基因促进同源染色体重组）杂交衍生的。这两个品系都比它们的亲本品系具有更高的耐盐性，而亲本品系比普通小麦背景中国春（CS）更耐盐。W 4909和W 4910小麦的基因组组成估计分别为1.9%和2.4%的非CS。使用AlTyphinaseGeneChip（R）Wheat Arrays鉴定在电导率（EQ为30 dS/m.基于AJDAJ 5和Ph '的表达多态性，在可能的情况下，将W 4909和W 4910中差异表达的基因归因于一个或另一个亲本系。对于W 4909和W 4910来说，Ph'亲本的表达多态性几乎是AJDAJ 5的两倍。W 4910中有9个转录本的超亲基因表达量显著高于或低于亲本。与对照相比，大多数表达多态性表现出非常低的表达水平，并定位于三个不同的染色体位置，2S，4L和2L。盐处理响应基因表达谱共同的所有五个测试线提供了一个简短的名单候选耐盐基因在小麦。从Ph ′转移的液泡膜水通道蛋白基因和归因于AJDAj 5的推定的钾通道蛋白基因被鉴定为W 4909和W 4910的组织耐盐性的候选基因。出版社：Elsevier爱尔兰Ltd.
Salt-tolerant wheat lines W4909 and W4910 were derived from a cross between AJDAJ5 (a disomic addition line carrying a pair of Eb chromosomes from Thinopyrum junceum) and Ph' (a line having the Ph' allele from Aegilops speltoides, which promotes homoeologous recombination). Both lines have greater salt tolerance than their parental lines, which are more salt-tolerant than the common wheat background, Chinese Spring (CS). Genomic constitution of W4909 and W4910 wheat has been estimated at 1.9% and 2.4% non-CS, respectively. AlTymetrix GeneChip (R) Wheat Arrays were used to identify differentially expressed genes in roots and leaves of the above-mentioned five lines under salt stress at electrical conductivity (EQ of 30 dS/m. Based on expression polymorphisms in AJDAJ5 and Ph', differentially expressed genes in W4909 and W4910 were attributed to one or the other parental lines when possible. The Ph' parent contributed nearly twice as many expression polymorphisms as AJDAJ5 to both W4909 and W4910. Nine transcripts in W4910 showed transgression gene expression significantly higher or lower than either parent. The majority of expression polymorphisms exhibited very low expression levels compared to the control and mapped to three distinct chromosomal locations, 2S, 4L and 2L. Salt treatment responsive gene expression profiles common to all five tested lines provide a short list of candidate salt-tolerance genes in wheat. A gene for tonoplast aquaporin that was transferred from Ph' and a gene for putative potassium channel protein that was attributable to AJDAj5 are identified as candidate genes for the tissue salt tolerance in W4909 and W4910. Published by Elsevier Ireland Ltd.