Expression of estrogen receptor related protein (p29) and estradiol binding in human arthritic synovium.
Expression of estrogen receptor related protein (p29) and estradiol binding in human arthritic synovium.
复制标题
人关节炎滑膜中雌激素受体相关蛋白 (p29) 的表达和雌二醇结合。
DOI：
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
J. Nishioka
中科院分区：
文献类型：
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作者：
T. Ushiyama;Koji Inoue;J. Nishioka
目标
我们研究了没有关节炎的受试者以及患有创伤性关节炎、骨关节炎和类风湿性关节炎的患者的人类滑膜组织中雌激素受体（ER）的表达和雌二醇的存在。
方法
使用针对 ER 相关蛋白 (p29) 的 ER-D5 单克隆抗体 (Mab)、针对核 ER 的 ER-ICA Mab 和抗雌二醇血清对滑膜组织进行免疫染色。荧光雌二醇用于证明雌二醇与 ER 的特异性结合。
结果
(1)p29主要存在于增生的滑膜衬里细胞和血管平滑肌中。 (2)外源性雌二醇与发炎滑膜中的滑膜细胞和下层巨噬细胞样细胞结合。 (3)在关节炎滑膜的增生性衬里细胞和巨噬细胞样细胞中也检测到内源性雌二醇。
结论
我们的研究证明了关节炎滑膜中 ER 的表达，并表明雌激素通过滑膜细胞以及下层巨噬细胞样细胞调节各种关节疾病中的局部炎症。
OBJECTIVE
We investigated the expression of estrogen receptors (ER) and the presence of estradiol in human synovial tissues from subjects without arthritis and patients with traumatic arthritis, osteoarthritis, and rheumatoid arthritis.
METHODS
Synovial tissues were immunostained using ER-D5 monoclonal antibody (Mab) directed toward an ER related protein (p29), ER-ICA Mab directed toward nuclear ER, and antiestradiol serum. Fluorescent estradiol was used to demonstrate specific estradiol binding to ER.
RESULTS
(1) p29 was present predominantly in hyperplastic synovial lining cells and smooth muscles of blood vessels. (2) Exogenous estradiol bound to synoviocytes and sublining macrophage-like cells in the inflamed synovium. (3) Endogenous estradiol was also detected in hyperplastic lining cells and macrophage-like cells in arthritic synovium.
CONCLUSION
Our study demonstrated the expression of ER in arthritic synovium and suggested that estrogen modulates local inflammation in various joint diseases via synoviocytes as well as sublining macrophage-like cells.