The two steps of vulval induction in Oscheius tipulae CEW1 recruit common regulators including a MEK kinase
The two steps of vulval induction in Oscheius tipulae CEW1 recruit common regulators including a MEK kinase
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DOI：
10.1016/j.ydbio.2003.09.010
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发表时间：
2004-01-01
影响因子：
2.7
通讯作者：
Félix, MA
中科院分区：
文献类型：
--
作者：
Dichtel-Danjoy, ML;Félix, MA
指定外阴前体细胞（VPC）命运的空间格局的细胞相互作用之间的线虫Oscheius tipulae CEW 1和秀丽隐杆线虫不同。在前者中，中心模式的命运是通过两个连续的诱导从性腺锚细胞，而在后者中，一个单一的诱导步骤的锚细胞（EGF-Ras-MAP激酶途径）可以作为一个形态发生素，并加强了横向信号之间的外阴前体（Notch途径）。我们对O. tipulae CEW 1.在这里，我们提出的突变体，具体影响外阴诱导机制。这些突变体的表型和上位性分析表明，两个外阴诱导步骤共享共同的组件，其中之一似乎是MEK激酶（S）。此外，诱导途径（包括MEK激酶）影响外阴前体细胞的能力，更显着的是，它们的分裂模式，以及，不管他们的外阴命运。最后，比较了C。elegans和O. tipulae CEW 1突出了两个物种之间外阴诱导机制的进化。(C)2003年爱思唯尔公司All rights reserved.
The cell interactions that specify the spatial pattern of vulval precursor cell (VPC) fates differ between the nematodes Oscheius tipulae CEW1 and Caenorhabditis elegans. In the former, the centered pattern of fates is obtained by two successive inductions from the gonadal anchor cell, whereas in the latter, a single inductive step by the anchor cell (EGF-Ras-MAP kinase pathway) can act as a morphogen and is reinforced by lateral signaling between the vulval precursors (Notch pathway). We performed a genetic screen for vulva mutants in O. tipulae CEW1. Here we present the mutants that specifically affect the vulval induction mechanisms. Phenotypic and epistatic analyses of these mutants show that both vulval induction steps share common components, one of which appears to be MEK kinase(s). Moreover, the inductive pathway (including MEK kinase) influences the competence of the vulval precursor cells and more strikingly their division pattern as well, irrespective of their vulval fate. Finally, a comparison of vulval mutant phenotypes obtained in C. elegans and O. tipulae CEW1 highlights the evolution of vulval induction mechanisms between the two species. (C) 2003 Elsevier Inc. All rights reserved.