Common promoter variant in cyclooxygenase-2 represses gene expression - Evidence of role in acute-phase inflammatory response
Common promoter variant in cyclooxygenase-2 represses gene expression - Evidence of role in acute-phase inflammatory response
复制标题
DOI：
10.1161/01.atv.0000030340.80207.c5
复制
发表时间：
2002-10-01
影响因子：
8.7
通讯作者：
Laurent, GJ
中科院分区：
文献类型：
--
作者：
Papafili, A;Hill, MR;Laurent, GJ
β-环氧合酶（考克斯）-2是与创伤和炎症相关的前列腺素类合成的关键调节酶。我们调查了考克斯-2基因的功能变异，可能会影响易感性diseases.Methods和Results-the启动子的考克斯-2的变异体在健康受试者通过使用聚合酶链反应为基础的方法进行了筛选。通过在人肺成纤维细胞中使用报告基因表达实验来研究启动子活性。对接受冠状动脉旁路移植术的患者进行基因分型，并测量与考克斯-2活性相关的血浆标记物。发现了一种常见的考克斯-2启动子变体-765 G>C，并显示>25%的英国健康受试者携带该变体。与-765G相比，-765C等位基因的启动子活性显著较低（低28+/-3%，P
Objective-Cyclooxygenase (COX)-2 is a key regulatory enzyme in the synthesis of prostanoids associated with trauma and inflammation. We investigated the COX-2 gene for functional variants that may influence susceptibility to disease.Methods and Results-The promoter of COX-2 was screened for variants in healthy subjects by use of polymerase chain reaction-based methods. Promoter activity was investigated by using reporter expression experiments in human lung fibroblasts. Patients undergoing coronary artery bypass graft surgery, with measurements of plasma markers linked to COX-2 activity, were genotyped for association studies. A common COX-2 promoter variant, -765G>C, was found and shown to be carried by >25% of a group of healthy UK subjects. The -765C allele had significantly lower promoter activity compared with -765G, basally (28+/-3% lower, P