Functional reconstruction of the non-human primate mandible using recombinant human bone morphogenetic protein-2
Functional reconstruction of the non-human primate mandible using recombinant human bone morphogenetic protein-2
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DOI：
10.1054/ijom.2001.0207
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发表时间：
2002-06-01
影响因子：
2.4
通讯作者：
Enomoto, S
中科院分区：
文献类型：
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作者：
Marukawa, E;Asahina, I;Enomoto, S
本研究的目的是评估重组人骨形态发生蛋白-2（rhBMP-2）在灵长类下颌骨节段性骨缺损中诱导再生骨的长期功能特性。在六只幼猴的下颌骨上制造30毫米的缺损，并用钛板固定下颌骨。然后将9毫克的rhBMP-2渗透到聚-D，L-乳酸-乙醇酸共聚物包被的明胶海绵（PGS）中，植入骨缺损处。术后20周将种植牙植入再生下颌骨，然后放置上层结构，并在种植牙植入后8周开始咀嚼力负荷。术后15周和30周以及咀嚼力负荷后4周和24周对骨形成和新骨质量进行放射学和组织学评估。切除的下颌骨通过 rhBMP-2 诱导的骨完全再生。加载后观察到新骨的良好重塑和巩固。这项研究表明，rhBMP-2 在大节段缺损中诱导的新骨能够维持并发挥功能至少一年。 rhBMP-2 诱导的骨再生有望成为未来的治疗方法，并且可能成为种植牙科和重建手术中自体骨移植的有效替代方案。
The purpose of this study was to evaluate the long-term functional properties of regenerated bone induced by recombinant human bone morphogenetic protein -2 (rhBMP-2) in segmental bone defects of primate mandibles. The 30-mm defects were created in the mandibles of six young monkeys and the mandibles were fixed with titanium plates. Then 9 mg of rhBMP-2 permeating a poly-D, L-lactic-co-glycolic acid-coated gelatin sponge (PGS) was implanted into the bone defect. Dental implants were placed into the regenerated mandible 20 weeks after surgery, then suprastructures were placed and masticatory force loading was begun 8 weeks after the insertion of the dental implants. Bone formation and the quality of new bone were evaluated radiologically and histologically at 15 and 30 weeks after surgery, and 4 and 24 weeks after masticatory force loading. The resected mandibles were completely regenerated with the rhBMP-2-induced bone. Excellent remodelling and consolidation of new bone were observed after loading. This study demonstrated that the new bone induced by rhBMP-2 in large segmental defects was maintained and functional for at least I year. Bone regeneration induced by rhBMP-2 holds promise as a future therapy and may be an effective alternative to autogenous bone grafts for implant dentistry and reconstructive surgery.