Immunochemical analysis of Taenia taeniaeformis antigens expressed in Escherichia coli
Immunochemical analysis of Taenia taeniaeformis antigens expressed in Escherichia coli
复制标题
大肠杆菌表达的带绦虫抗原的免疫化学分析
作者：
D. Bowtell;R. Saint;M. Rickard;G. Mitchell
先前我们报道了几种表达带绦虫抗原片段为β-半乳糖苷酶融合蛋白的大肠杆菌克隆的分离（Bowtell, Saint, Rickard & Mitchell, 1984）。在这里，我们描述了从幼虫cDNA文库中分离到其他表达抗原的克隆，并将这些克隆分配到7个抗原家族。这些细胞是用兔多特异性抗血清分离出来的。由于该血清能够与大量抗原发生反应，因此开发快速确定克隆抗原基因对应的原生带绦虫分子的技术非常重要。其中包括用融合蛋白对家兔进行主动免疫，以及对固定化融合蛋白进行亲和纯化的几种技术。还评估了抗原阳性克隆与感染相关寄生虫的人血清的反应性。最后，几种融合蛋白对小鼠的免疫不能保护小鼠免受随后的感染，尽管先前确定的候选宿主保护抗原（Bowtell, Mitchell, Anders, Lightowlers & Rickard, 1983）尚未在表达文库中确定。
SUMMARY Previously we reported the isolation of several Escherichia coli clones expressing fragments of Taenia taeniaeformis antigens as β-galactosidase fused proteins (Bowtell, Saint, Rickard & Mitchell, 1984). Here we describe the isolation of additional antigen-expressing clones from a larval cDNA library and the assignment of these clones to 7 antigen families. These were isolated with a polyspecific rabbit antiserum raised to the oncosphere. Since this serum was capable of reacting with a large number of antigens, it was important to develop techniques for rapidly determining the identity of the native T. taeniaeformis molecule corresponding to a cloned antigen gene. These included active immunization of rabbits with fused proteins and several techniques involving affinity purification on immobilized fused proteins. The reactivity of the antigen-positive clones with sera from humans infected with related parasites was also assessed. Finally, immunization of mice with several fused proteins failed to protect against subsequent infection, although antigens previously identified as candidate host-protective antigens (Bowtell, Mitchell, Anders, Lightowlers & Rickard, 1983) have yet to be identified in the expression library.
绦虫后绦虫 (ECF-Tt) 中嗜酸性粒细胞趋化因子的分离和部分表征。
DOI：
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发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Potter,KA;Leid,RW
通讯作者：
Leid,RW
DOI：
10.1073/pnas.80.5.1194
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
YOUNG, RA;DAVIS, RW
通讯作者：
DAVIS, RW
T 细胞对 Mls 位点不同刺激细胞反应的免疫生物学。
DOI：
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发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Katz,ME;Tite,JP;JanewayJr,CA
通讯作者：
JanewayJr,CA