Cytotoxic and anti-angiogenic properties of the stem bark extract of Sandoricum koetjape.
Cytotoxic and anti-angiogenic properties of the stem bark extract of Sandoricum koetjape.
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DOI：
10.3923/ijcr.2009.105.114
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发表时间：
2009-01-01
期刊：
影响因子：
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通讯作者：
Abdul Majid, A. M. S.
中科院分区：
文献类型：
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作者：
Aisha, A. F. A.;Sahib, H. B.;Abdul Majid, A. M. S.
采用体外细胞毒性实验和抗血管生成实验研究了山茱萸茎皮正己烷提取物的体外细胞毒性和抗血管生成作用。采用细胞增殖试验研究了2，3-二-（2-甲氧基-4-硝基-5-磺基苯基）-2H-四唑-5-甲酰苯胺（XTT）对人脐静脉内皮细胞（HUVEC）以及结肠癌和正常细胞系HCT-116、HT-29和CCD-18 CO的细胞毒性。采用大鼠主动脉环实验研究其抗血管生成作用。在100 g mL-1时，提取物对大鼠主动脉环血管生长的抑制率为945.5%。浸提液还显示出对所有试验细胞系的剂量依赖性生长抑制，对HCT-116、HUVEC、CCD-18 CO和HT-29的IC 50值分别为14、23、50和52 g mL-1。在50 g mL-1时，提取物通过诱导半胱天冬酶3和7活性而有效诱导HCT-116结肠癌细胞系的凋亡细胞死亡。结果表明，S. Koetjape对结肠癌细胞系具有抗血管生成和凋亡特性，使其成为进一步研究的良好候选物。
Cytotoxic and anti-angiogenic properties of n-hexane extract of Sandoricum koetjape stem bark were investigated in vitro. 2,3-bis-(2-methoxy-4-nitro- 5-sulfophenyl)-2H-tetrazolium- 5-carboxanilide (XTT) cell proliferation assay was used to study the cytotoxic properties on Human Umbilical Vein Endothelial Cell (HUVEC) and both colon cancer and normal cell lines; HCT-116, HT-29 and CCD-18CO. Rat aorta ring assay was used to study the anti-angiogenic properties of the extract. At 100 g mL-1, the extract showed 945.5% inhibition of the outgrowth of the blood vessels from the rat aorta rings. The extract also showed a dose dependent growth inhibition of all tested cell lines, IC50 values against HCT-116, HUVEC, CCD-18CO and HT-29 were 14, 23, 50 and 52 g mL-1, respectively. At 50 g mL-1, the extract had potently induced apoptotic cell death of HCT-116 colon cancer cell line by inducing caspases 3 and 7 activity. These results showed that n-hexane extract of S. koetjape possess both anti-angiogenic and apoptotic properties on colon cancer cell lines making it a good candidate for further studies.