Laser-based patterning for fluidic devices in nitrocellulose
Laser-based patterning for fluidic devices in nitrocellulose
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DOI：
10.1063/1.4919629
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发表时间：
2015-03-01
期刊：
影响因子：
3.2
通讯作者：
Sones, Collin L.
中科院分区：
文献类型：
--
作者：
He, Peijun J. W.;Katis, Ioannis N.;Sones, Collin L.
在这份报告中，我们展示了一个简单的和低成本的方法，可以重复用于制造微流体装置在硝化纤维素。流体图案通过基于激光的直写技术产生，该技术诱导先前浸渍在硝化纤维素中的光聚合物的聚合。所得结构形成疏水屏障，其延伸穿过硝化纤维素的厚度并限定互连的亲水流体流动模式。我们的实验结果表明，使用这种方法，可以使用形成尺寸类似于60 μ m的通道壁的疏水屏障来实现横向尺寸类似于100 μ m的微流体通道;这两个值都比那些值小得多。使用制造基于硝化纤维素的流体装置中使用的其他当前技术可以实现。一个简单的网格图案的硝化纤维素装置，然后用于检测C-反应蛋白通过夹心酶联免疫吸附试验，这是一个有用的原理验证实验。(C)2015 AIP Publishing LLC.
In this report, we demonstrate a simple and low cost method that can be reproducibly used for fabrication of microfluidic devices in nitrocellulose. The fluidic patterns are created via a laser-based direct-write technique that induces polymerisation of a photo-polymer previously impregnated in the nitrocellulose. The resulting structures form hydrophobic barriers that extend through the thickness of the nitrocellulose and define an interconnected hydrophilic fluidic-flow pattern. Our experimental results show that using this method it is possible to achieve microfluidic channels with lateral dimensions of similar to 100 mu m using hydrophobic barriers that form the channel walls with dimensions of similar to 60 mu m; both of these values are considerably smaller than those that can be achieved with other current techniques used in the fabrication of nitrocellulose-based fluidic devices. A simple grid patterned nitrocellulose device was then used for the detection of C-reactive protein via a sandwich enzyme-linked immunosorbent assay, which served as a useful proof-of-principle experiment. (C) 2015 AIP Publishing LLC.