The iron regulatory protein can determine the effectiveness of 5-aminolevulinic acid in inducing protoporphyrin IX in human primary skin fibroblasts.
The iron regulatory protein can determine the effectiveness of 5-aminolevulinic acid in inducing protoporphyrin IX in human primary skin fibroblasts.
复制标题
铁调节蛋白可以确定 5-氨基乙酰丙酸在人原代皮肤成纤维细胞中诱导原卟啉 IX 的有效性。
DOI：
10.1046/j.1523-1747.1999.00556.x
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
R. Tyrrell
中科院分区：
文献类型：
--
作者：
C. Pourzand;O. Reelfs;E. Kvam;R. Tyrrell
5-氨基乙酰丙酸（ALA）可提高细胞内内源性光敏剂原卟啉IX（PPIX）的水平。我们研究了关键参数的影响，如细胞的生长状态和细胞内铁的可用性在调节PPIX的水平，在人原代培养的皮肤成纤维细胞（FEK 4）保持在指数生长或生长停滞期，用ALA治疗后。添加ALA指数增长的细胞增加了PPIX的水平6倍，相对于对照细胞，然而，在生长停滞的细胞中，相同的治疗增加了PPIX的水平高达34倍。同时加入亲水性铁螯合剂Desferal和ALA，使PPIX的水平在生长细胞中提高了47倍，在生长停滞细胞中提高了42倍，这表明铁在后一种条件下是有限的。PPIX增强对游离有效铁水平的严格依赖性通过带移测定中铁调节蛋白的活化水平来检查。该分析显示，生长停滞细胞中铁调节蛋白的基础水平比生长中的细胞高6倍，反映了指数生长细胞中游离可用铁库的影响。有趣的是，在生长和生长停滞细胞中PPIX的基础水平浓度之间发现了相同的比率。我们建议，铁调节蛋白激活可以作为一个标记，用于开发光动力治疗方案，因为它确定了细胞和组织的倾向，积累PPIX，因此很可能预测这种疗法的有效性。
The level of endogenous photosensitiser, protoporphyrin IX (PPIX), can be enhanced in the cells by 5-aminolevulinic acid (ALA). We investigated the effect of critical parameters such as growth state of the cells and availability of intracellular iron in modulating the level of PPIX, in human primary cultured skin fibroblasts (FEK4) maintained either in exponentially growing or growth-arrested phase, following treatment with ALA. The addition of ALA to exponentially growing cells increased the level of PPIX 6-fold relative to control cells; however, in growth-arrested cells the same treatment increased the level of PPIX up to 34-fold. The simultaneous addition of the hydrophilic iron-chelator Desferal with ALA, boosted the level of PPIX up to 47-fold in growing cells and up to 42-fold in growth-arrested cells, suggesting that iron is limiting under the latter conditions. The strict dependence of PPIX enhancement on free available iron levels was examined by the level of activation of iron regulatory protein in band shift assays. This analysis revealed that the basal level of iron regulatory protein in growth-arrested cells was 6-fold higher than in growing cells, reflecting the influence of the free available iron pool in exponentially growing cells. Interestingly, the same ratio was found between the basal level concentration of PPIX in growing and growth-arrested cells. We propose that iron regulatory protein activation could serve as a marker for developing photodynamic therapy protocols because it identifies cells and tissues with a propensity to accumulate PPIX and it is therefore likely to predict the effectiveness of such therapies.
用5-氨基烯酸酸诱导的卟啉的细胞光铸造的机械研究。
影响因子：
8.8
作者：
Iinuma, S.;Farshi, S. S.;Ortel, B.;Hasan, T.
通讯作者：
Hasan, T.