CONFORMATION OF MACROMOLECULES IN THE GAS-PHASE - USE OF MATRIX-ASSISTED LASER-DESORPTION METHODS IN ION CHROMATOGRAPHY
CONFORMATION OF MACROMOLECULES IN THE GAS-PHASE - USE OF MATRIX-ASSISTED LASER-DESORPTION METHODS IN ION CHROMATOGRAPHY
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DOI：
10.1126/science.267.5203.1483
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发表时间：
1995-03-10
期刊：
影响因子：
56.9
通讯作者：
BOWERS, MT
中科院分区：
文献类型：
--
作者：
VON HELDEN, G;WYTTENBACH, T;BOWERS, MT
Conformational data for macromolecules in the gas phase have been obtained by the coupling of a matrix-assisted laser desorption ion source to an ion chromatograph. A series of polyethylene glycol (PEG) polymers ''cationized'' (converted to a cation) by sodium ions (Na(+)PEG9 to Na(+)PEG19) and a protonated neurotransmitter protein, bradykinin, were studied. Mobilities of Na(+)PEG9 to Na(+)PEG19 are reported. Detailed modeling of Na(+)PEG9 with molecular mechanics methods indicates that the lowest energy structure has the Na+ ion ''solvated'' by the polymer chain with seven oxygen atoms as nearest neighbors. The agreement between the model and experiment is within 1 percent for Na(+)PEG9, Na(+)PEG13, and Na(+)PEG17, giving strong support to both the method and the deduced structures. Similar agreement was obtained in initial studies that modeled experimental data for arginine-protonated bradykinin.
