Dynamic patterns of USPIO enhancement can be observed in macrophages after ischemic brain damage
Dynamic patterns of USPIO enhancement can be observed in macrophages after ischemic brain damage
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DOI：
10.1002/mrm.1290
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发表时间：
2001-11-01
影响因子：
3.3
通讯作者：
Rudin, M
中科院分区：
文献类型：
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作者：
Rausch, M;Sauter, A;Rudin, M
单核吞噬系统（MPS）的细胞经常在缺血组织附近或内部发现，并可能加重细胞损伤。因此，可视化这些细胞将允许从完整组织中区分推定的受影响组织。实验MR1研究表明，葡聚糖包覆氧化铁（USPIO）的超小颗粒被内化到MPS细胞中。为了检验这种细胞标记方法是否也适用于脑梗死，我们在大脑中动脉永久性闭塞（pMCAO）后5.5 h给药USPIOs。在前2天，USPIO优先出现在病变内的斑块和周围区域。第4天，USPIOs在病灶核心内扩大。在第7天，它们主要在边界地区被发现。组织学分析显示梗死组织内有大量含铁颗粒的巨噬细胞。因此，我们得出结论，使用USPIOs监测局灶性脑缺血后MPS活性是可能的。(C) 2001 Wiley-Liss, Inc。
Cells of the mononuclear phagocytotic system (MPS) are often found near to or within ischemic tissue and can potentially aggravate cellular damage. Hence, visualization of those cells would allow demarcation of putatively affected from intact tissue. Experimental MR1 studies have shown that ultrasmall particles of dextran-coated iron oxide (USPIO) are internalized into cells of the MPS. To test if this cell tagging method may be also applied to cerebral infarction, USPIOs were administered to Fisher rats 5.5 h after permanent occlusion of the middle cerebral artery (pMCAO). During the first 2 days USPIO were preferentially found in patches within the lesion and in surrounding areas. On day 4, USPIOs expanded within the core of the lesion. On day 7 they were found predominantly within the boundary area. Histological analysis showed large populations of macrophages containing iron particles in the infarcted tissue. We conclude, therefore, that it is possible to monitor MPS activity after focal cerebral ischemia using USPIOs. (C) 2001 Wiley-Liss, Inc.