Inhibition of connexin 43 alters Shh and Bmp-2 expression patterns in embryonic mouse tongue
Inhibition of connexin 43 alters Shh and Bmp-2 expression patterns in embryonic mouse tongue
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DOI：
10.1007/s00441-005-1091-y
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发表时间：
2005-06-01
影响因子：
3.6
通讯作者：
Jung, HS
中科院分区：
文献类型：
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作者：
Kim, JY;Cho, SW;Jung, HS
真菌状乳头的形态发生通过上皮-间质相互作用以固定模式发生在哺乳动物舌背表面。这些相互作用被认为是通过细胞间通讯实现的。间隙连接可以在许多发育组织中观察到，并且被认为参与多种功能，包括细胞增殖、分化和凋亡的调节。在这里，我们证明间隙连接蛋白 Connexin 43 (Cx43) 的表达与菌状乳头的发育显着相关，其表现出与其他上皮附属物的发育相似的模式形成和形态发生。针对 Cx43 的反义寡脱氧核苷酸 (AS-ODN) 用于评估 Cx43 的发育功能。 Cx43 抑制会破坏信号分子的表达模式。有趣的是，Shh（确定菌状乳头间距模式的关键分子）的表达模式在 Cx43 AS-ODN 处理后受到干扰。我们还尝试通过将 NOGGIN 蛋白应用于舌培养物来确定 Bmp-2 的功能。我们的结果表明，通过 Cx43 的上游调控控制着真菌状乳头形态发生和模式形成的 Shh 和 Bmp-2 途径。
The morphogenesis of fungiform papillae occurs in a stereotyped pattern on the dorsal surface of the mammalian tongue via epithelial-mesenchymal interactions. These interactions are thought to be achieved via intercellular communication. Gap junctions can be observed in many developing tissues and have been suggested to participate in a variety of functions, including the regulation of cell proliferation, differentiation, and apoptosis. Here, we demonstrate that the expression of Connexin 43 (Cx43), a gap junction protein, is correlated significantly with the development of fungiform papillae, which exhibit a pattern formation and morphogenesis similar to the development of other epithelial appendages. Antisense-oligodeoxynucleotide (AS-ODN) against Cx43 was used to assess the developmental functions of Cx43. The expression patterns of the signaling molecules were disrupted by Cx43 inhibition. Interestingly, the expression patterns of Shh, a key molecule in the determination of the spacing patterns of fungiform papillae, were disturbed after treatment with Cx43 AS-ODN. We have also attempted to determine the functions of Bmp-2 by applying NOGGIN protein to tongue cultures. Our results indicate that upstream regulation via Cx43 controls the Shh and Bmp-2 pathways for the morphogenesis and pattern formation of fungiform papillae.