Ara h 2 is the dominant peanut allergen despite similarities with Ara h 6
Ara h 2 is the dominant peanut allergen despite similarities with Ara h 6
复制标题
DOI：
10.1016/j.jaci.2020.03.026
复制
发表时间：
2020-09-01
影响因子：
14.2
通讯作者：
Santos, Alexandra F.
中科院分区：
文献类型：
--
作者：
Hemmings, Oliver;Du Toit, George;Santos, Alexandra F.
背景：花生2（Arah 2）特异性IgE是目前诊断花生过敏的最佳血清学标志物. Ara h 6与Ara h 2具有60%的同源性和多表位性。目的：探讨Ara h 2和Ara h 6在花生过敏诊断中的应用价值和相对重要性。该队列包括对花生过敏的儿童，对花生过敏但耐受的儿童，以及对花生既不过敏也不过敏的儿童。通过使用ImmunoCAP对花生和个体过敏原的特异性IgE水平进行定量。免疫CAP抑制实验和肥大细胞活化试验响应于Ara h 2和Ara h 6进行。结果：Ara h 2特异性IgE和Ara h 6特异性IgE对花生过敏的诊断准确率最高，其次为花生特异性IgE和其他过敏原。大多数花生过敏患者对Ara h 2和Ara h 6均致敏。Ara h 2比Ara h 6更能降低Ara h 2特异性IgE结合（P < .001），而Ara h 2和Ara h 6对Ara h 6特异性IgE结合的抑制程度相似（P = .432）。在肥大细胞活化试验中，阿糖胞苷h 2诱导显着更大的最大反应性（P = 0.001）和较低的半最大有效浓度（P = 0.002）比阿糖胞苷h 6测试cosensitized individual.Conclusions：阿糖胞苷h 2特异性IgE和阿糖胞苷h 6特异性IgE提供了最大的准确性诊断花生过敏。阿糖胞苷h 2是在英国花生过敏的主要conglutin，尽管一定程度的交叉反应与阿糖胞苷h 6。
Background: Arachis hypogaea 2 (Ara h 2)-specific IgE is to date the best serologic marker to diagnose peanut allergy. Ara h 6 shares approximately 60% sequence identity and multiple epitopes with Ara h 2.Objective: Our aim was to assess the diagnostic utility and relative importance of Ara h 2 and Ara h 6 in peanut allergy.Methods: A cohort 100 of children was studied. The cohort included chidren who had peanut allergy, children who were sensitized to but tolerant of peanut, and children who were neither sensitized nor allergic to peanut. Levels of specific IgE to peanut and individual allergens were quantified by using ImmunoCAP. ImmunoCAP inhibition experiments and mast cell activation tests in response to both Ara h 2 and Ara h 6 were performed. Statistical analyses were done using SPSS version 14 and Prism version 7 software.Results: Ara h 2-specific IgE and Ara h 6-specific IgE showed the greatest diagnostic accuracy for peanut allergy when compared with specific IgE to peanut and other peanut allergens. Most patients with peanut allergy were sensitized to both Ara h 2 and Ara h 6. Ara h 2 reduced Ara h 2-specific IgE binding more than Ara h 6 did (P < .001), whereas Ara h 6-specific IgE binding was inhibited to a similar degree by Ara h 2 and Ara h 6 (P = .432). In the mast cell activation test, Ara h 2 induced significantly greater maximal reactivity (P = .001) and a lower half maximal effective concentration (P = .002) than did Ara h 6 when testing cosensitized individuals.Conclusions: Ara h 2-specific IgE and Ara h 6-specific IgE provide the greatest accuracy to diagnose peanut allergy. Ara h 2 is the dominant conglutin in peanut allergy in the United Kingdom, despite a degree of cross-reactivity with Ara h 6.