Using barrier screens to characterize mosquito composition, flight activity, and abdominal status in South Lampung, Indonesia
Using barrier screens to characterize mosquito composition, flight activity, and abdominal status in South Lampung, Indonesia
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DOI：
10.1186/s13071-018-3031-1
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发表时间：
2018-07-31
影响因子：
3.2
通讯作者：
Lobo, Neil F.
中科院分区：
文献类型：
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作者：
Davidson, Jenna R.;Sukowati, Supratman;Lobo, Neil F.
背景：蚊子采样方法针对蚊子行为的不同方面，并受到陷阱和地点特定偏差的影响。开发了屏障屏蔽法，并对自由飞行、吸血和寻找寄主的蚊子进行了采样。在一项初步研究中，这种方法在获取在室外幼虫栖息地和采集血粉的地点之间飞行的蚊子样本方面是有用的。然而，在初步研究期间，在印度尼西亚收集了数量相对较少的以血液为食的按蚊。在印度尼西亚南楠榜推广了抽样方法，以便能够收集以血液为食的蚊子。本研究旨在利用屏障屏障拦截在人类栖息地和幼虫栖息地之间飞行的蚊子，并对蚊子的组成、飞行特征(方向、高度和时间)、腹部状态和产次进行分析。结果：屏障屏障拦截了南楠榜15种不同种类的蚊子：8种按蚊。和7个库蚊(Culex spp.)楠榜南部不同村落的物种组成各不相同。约15%的按蚊。被捕获的是吸血的，其中28.2%的被测试者以人为食。这是印度尼西亚首次使用屏障收集到以血液为食的按蚊。确定的血粉包括牛、狗、山羊和人，以及混合血粉。未喂食蚊子的活动。采集到的主要媒介亚毛虫在20：00-12：00 h达到高峰，活动缓慢下降，直到18：00 h。Sundaicus，与An.亚比图斯。其他物种表现出不同的活动高峰时间，较早的活动是总的趋势。采集的按蚊中，55.5%是在0.5m以下捕获的，83.9%是在离地1m处捕获的。胎次解剖使物种的年龄结构成为可能，这揭示了物种特有的特征，如未分娩的和未分娩的。黑夜比黑夜早些时候更活跃。结论：屏蔽法是一种有效的蚊虫采样方法，可用于了解当地蚊虫种类组成、飞行特征(方向、高度和时间)、腹部状况和产次。
Background: Mosquito sampling methods target different aspects of mosquito behavior and are subject to trap and location specific biases. The barrier screen sampling method was developed and tested to sample free-flying, blood-fed, and host-seeking mosquitoes. During a pilot study, this method was useful in obtaining an unbiased sample of mosquitoes flying between outdoor larval habitats, and sites where blood meals were obtained. However, a relatively small number of blood-fed Anopheles mosquitoes were collected in Indonesia during the pilot study. The sampling method was extended in South Lampung, Indonesia, to enable the collection of blood-fed mosquitoes. This study aimed to intercept mosquitoes flying between human habitations and larval habitats with a barrier screen and to characterize mosquito composition, flight characteristics (direction, height and time), abdominal status, and parity.Results: Barrier screens intercepted 15 different mosquito species in South Lampung: eight Anopheles spp. and seven Culex spp. Species compositions varied among the villages in South Lampung. About 15% of Anopheles spp. caught were blood-fed, of which 28.2% of those tested had fed on humans. This is the first time human blood-fed anophelines have been collected in Indonesia using barrier screens. Blood meals identified included cow, dog, goat, and human, as well as mixed blood meals. Activity of unfed An. subpictus, the primary vector collected, flying towards human habitations peaked between 20:00-12:00 h, with a slow decline in activity until 18:00 h. Unfed and fed An. sundaicus, had a different activity profile compared to An. subpictus. Other species demonstrated varied peak activity times, with earlier activity occurring as a general trend. For the Anopheles mosquitoes collected, 55.5% were collected below 0.5 m and 83.9% were captured resting < 1 m from the ground. Parity dissections enabled age structure by species, which revealed species-specific traits such as nulliparous An. subpictus being more active early in the night relative to An. sundaicus.Conclusions: This study demonstrates that barrier screens are an effective mosquito sampling method that can be used to gain insights into local mosquito species composition, flight characteristics (direction, height and time), abdominal status, and parity.