MECHANISM OF SYNTHESIS OF VACCINIA VIRUS DOUBLE-STRANDED RIBONUCLEIC ACID IN-VIVO AND IN-VITRO
MECHANISM OF SYNTHESIS OF VACCINIA VIRUS DOUBLE-STRANDED RIBONUCLEIC ACID IN-VIVO AND IN-VITRO
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DOI：
10.1128/jvi.7.1.71-76.1971
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发表时间：
1971-01-01
影响因子：
5.4
通讯作者：
COLBY, C
中科院分区：
文献类型：
--
作者：
COLBY, C
The synthesis of vaccinia virus double-stranded ribonucleic acid (RNA) in infected HeLa cells was sensitive to actinomycin D, suggesting that a deoxyribonucleic acid dependent reaction is involved. Some double-stranded RNA was made in the presence of cytosine arabinoside in infected cells. Double-stranded and complementary RNA were synthesized in vitro by using vaccinia cores. These two observations indicate that some of the double-stranded RNA is read from “early” genes. The double-stranded RNA synthesized in vitro had the same properties as that made in vivo. At least 70% of the double-stranded RNA made in vivo was in ribonuclease-resistant form prior to sodium dodecyl sulfate-phenol extraction. In addition, there was a complementary RNA in infected cells which could be converted to double-stranded RNA by annealing.
