Within- and between-subject variation in semen parameters in infertile men and normal semen donors
Within- and between-subject variation in semen parameters in infertile men and normal semen donors
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DOI：
10.1016/j.fertnstert.2005.06.048
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发表时间：
2006-01-01
影响因子：
6.7
通讯作者：
Keel, BA
中科院分区：
文献类型：
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作者：
Keel, BA
目的：了解不育男性和正常男性精液参数在受试者内和受试者间的差异。设计：对74例不育男性和65例有5次或5次以上射精量的正常男性进行回顾性分析。地点：临床男科实验室。受试者(S)：到不育门诊进行精液分析的男性和提供捐精者精液用于冷冻保存的正常男性。干预(S)：无。主要结果衡量(S)：受试者内和受试者间的变异系数，计算精子数、精子活动力和精子速度、精液量、精液量、精子数、精子活动力和精子速度。结果(S)：受者射精数为5~20个(共479个)，供者为5~136个(共2 043个)。捐献者的精子数量、活动率和速度在统计学上更高，而患者的精液量则显著更大。患者和供者的CVW精子数最多(分别为54.2%和45.8%)，精子速度最低(分别为20.1%和19.9%)。患者和供者的CVB在所有参数上都明显高于CVW。与患者相比，供者的CVW较低，CVB较高。CVW不受年龄和禁欲年限的影响。超低温保存导致变异性显著增加。组内相关性表明，至少需要三个精液样本才能达到可靠性。结论：这些数据表明，患者和健康捐赠者的精子参数，特别是精子数量，在受试者内和受试者之间存在较大差异，进一步证实了在将男性定性为正常或不育之前，需要测量多次射精。
Objective: To determine within- and between-subject variation of semen parameters in infertile and normal men.Design: Retrospective analysis of 74 infertile men and 65 normal men producing five or more ejaculates each.Setting: Clinical andrology laboratory.Subject(s): Men presenting to an infertility clinic for semen analysis and normal men providing donor semen for cryopreservation.Intervention(S): None.Main Outcome Measure(s): Within-subject coefficients of variation (CVw) and between-subject coefficients of variation (CVb), calculated for sperm count, motility, and velocity, semen volume, and motility and velocity after cryopreservation.Result(s): The number of ejaculates ranged 5-20 in patients (479 total) and 5-136 in donors (2,043 total). Sperm counts, motility, and velocity were statistically greater in donors, whereas semen volume was significantly greater in patients. The CVw in patients and donors was greatest in sperm counts (54.2% and 45.8%, respectively) and lowest in velocity (20.1% and 19.9%, respectively). The CVb in patients and donors was markedly higher than CVw for all parameters in both patients and donors. The CVw was lower, and the CVb was higher, in donors compared with patients. The CVw was not affected by age of the individual or abstinence period. Cryopreservation resulted in a marked increase in variability. Intraclass correlations indicated that a minimum of three semen samples were required to achieve reliability.Conclusion(s): These data indicate large within- and between-subject variation in sperm parameters, especially sperm count, in both patients and healthy donors, and further substantiate the need for measurement of multiple ejaculates before characterizing a man as normal or infertile.