A novel adenovirus of Western lowland gorillas (Gorilla gorilla gorilla).
A novel adenovirus of Western lowland gorillas (Gorilla gorilla gorilla).
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DOI：
10.1186/1743-422x-7-303
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发表时间：
2010-11-05
期刊：
影响因子：
4.8
通讯作者：
Ehlers B
中科院分区：
文献类型：
--
作者：
Wevers D;Leendertz FH;Scuda N;Boesch C;Robbins MM;Head J;Ludwig C;Kühn J;Ehlers B
腺病毒（AdV）广泛感染包括多种灵长类在内的脊椎动物宿主。我们通过细胞培养、电镜和PCR等方法从圈养大猩猩粪便中分离到一种新的腺病毒。从感染培养物的上清液中，我们扩增DNA聚合酶（DPOL），前末端蛋白（pTP）和六邻体基因序列与通用泛灵长类动物腺病毒PCR检测。通过2 - 10 kb长度的长距离PCR扩增中间的序列，得到15.6 kb的最终序列。系统发育分析将新的大猩猩AdV置于属于人腺病毒B（HAdV-B）物种的灵长类AdV簇中。根据所分析的基因，其在簇内的位置是可变的。为了进一步阐明其起源，对野生大猩猩的粪便样本进行了分析。检测到AdV六邻体序列，其指示三种不同且新颖的大猩猩HAdV-B病毒，其中一种病毒与从圈养大猩猩分离的新颖AdV几乎相同。这表明发现的病毒是自然感染大猩猩的一组HAdV-B病毒的成员。大猩猩，黑猩猩，倭黑猩猩和人类的HAdV-B物种内的AdV的混合系统发育簇表明，主机开关可能是人类和非人灵长类HAdV-B病毒的进化的一个组成部分。
Adenoviruses (AdV) broadly infect vertebrate hosts including a variety of primates. We identified a novel AdV in the feces of captive gorillas by isolation in cell culture, electron microscopy and PCR. From the supernatants of infected cultures we amplified DNA polymerase (DPOL), preterminal protein (pTP) and hexon gene sequences with generic pan primate AdV PCR assays. The sequences in-between were amplified by long-distance PCRs of 2 - 10 kb length, resulting in a final sequence of 15.6 kb. Phylogenetic analysis placed the novel gorilla AdV into a cluster of primate AdVs belonging to the species Human adenovirus B (HAdV-B). Depending on the analyzed gene, its position within the cluster was variable. To further elucidate its origin, feces samples of wild gorillas were analyzed. AdV hexon sequences were detected which are indicative for three distinct and novel gorilla HAdV-B viruses, among them a virus nearly identical to the novel AdV isolated from captive gorillas. This shows that the discovered virus is a member of a group of HAdV-B viruses that naturally infect gorillas. The mixed phylogenetic clusters of gorilla, chimpanzee, bonobo and human AdVs within the HAdV-B species indicate that host switches may have been a component of the evolution of human and non-human primate HAdV-B viruses.