Relaxin secretion by human granulosa cell culture is predictive of in-vitro fertilization-embryo transfer success.
Relaxin secretion by human granulosa cell culture is predictive of in-vitro fertilization-embryo transfer success.
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人颗粒细胞培养物分泌的松弛素可预测体外受精-胚胎移植的成功。
DOI：
10.1093/humrep/14.2.338
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
VandeVoort,CA
中科院分区：
文献类型：
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作者：
Stewart,DR;VandeVoort,CA
我们已经开发了一种用于人黄体化颗粒细胞的细胞培养系统，其支持雌激素、孕酮和松弛素的及时和动态分泌，其模式模拟月经周期的黄体期期间这些激素的血清浓度。在研究的69名患者中，培养细胞分泌的松弛素量存在很大差异。由于松弛素的产生通常在培养的第10天达到最大，因此在该时间点进行比较。结果表明，大多数妊娠发生在体外松弛素分泌较高的患者中。第10天松弛素>800 pg/ml的所有周期都有足月妊娠，而松弛素<200 pg/ml的周期只有13%有足月妊娠。来自供体/受体周期的有限数量的周期未显示类似的结果。类固醇浓度不能预测怀孕。这些结果表明，松弛素的体外产生是体外受精（IVF）-胚胎移植周期中植入成功的预测因素。这支持了松弛素可能参与着床的假设，松弛素浓度降低可能是IVF后妊娠率低的部分原因。
We have developed a cell culture system for human luteinizing granulosa cells which supports the timely and dynamic secretion of oestrogen, progesterone and relaxin in patterns that mimic serum concentrations of these hormones during the luteal phase of the menstrual cycle. There was a wide variation in the amount of relaxin secreted by the cultured cells for the 69 patients studied. As relaxin production was generally maximal by day 10 of culture, comparisons were made at this time point. It was observed that most of the conceptions occurred in patients with higher relaxin secretionin vitro. All cycles with relaxin >800 pg/ml on day 10 had a term pregnancy while only 13% of cycles with relaxin <200 pg/ml had term pregnancies. A limited number of cycles from donor/recipient cycles did not show similar results. Steroid concentrations were not predictive of conception. These results demonstrated that in-vitro production of relaxin is predictive of implantation success in in-vitro fertilization (IVF)–embryo transfer cycles. This supports the hypothesis that relaxin may be involved in implantation and that lowered relaxin concentrations may be a partial cause of poor pregnancy rates after IVF.