Priming of CD4+ T cells specific for conserved regions of human immunodeficiency virus glycoprotein gp120 in humans immunized with a recombinant envelope protein.
Priming of CD4+ T cells specific for conserved regions of human immunodeficiency virus glycoprotein gp120 in humans immunized with a recombinant envelope protein.
复制标题
在用重组包膜蛋白免疫的人体中，启动对人类免疫缺陷病毒糖蛋白 gp120 保守区域具有特异性的 CD4 T 细胞。
DOI：
--
复制
发表时间：
1990
影响因子：
11.1
通讯作者：
T. Staehelin
中科院分区：
文献类型：
--
作者：
S. Abrignani;D. Montagna;M. Jeannet;J. Wintsch;N. Haigwood;J. Shuster;K. Steimer;A. Cruchaud;T. Staehelin
以非糖基化的人类免疫缺陷病毒(HIV)1糖蛋白gp120(env2-3)为佐剂，免疫HIV血清阴性的健康志愿者。在所有志愿者中，三次50微克的Env2-3注射诱导了针对天然糖基化gp120保守区域的CD4+T细胞的启动。此外，我们发现几个主要的组织相容性复合体II类(DR)等位基因可以作为限制分子将gp120的保守表位呈递给T细胞，这意味着在很大一部分人群中可以获得对这些表位的T细胞应答。考虑到HIV感染者体内缺乏针对HIV蛋白保守表位的CD4+T细胞，以及CD4+T细胞可能在发展对艾滋病的保护性免疫中发挥的作用，启动这种细胞的可能性尤为重要。
A nonglycosylated denatured form of human immunodeficiency virus (HIV) 1 glycoprotein gp120 (Env 2-3), which does not bind to CD4, was used with muramyl tripeptide as adjuvant to immunize HIV-seronegative healthy volunteers. In all the volunteers, three 50-micrograms injections of Env 2-3 induced priming of CD4+ T cells specific for conserved regions of the native glycosylated gp120. Moreover, we found that several major histocompatibility complex class II (DR) alleles can function as restriction molecules for presentation of conserved epitopes of gp120 to T cells, implying that a T-cell response to these epitopes can be obtained in a large fraction of the population. The possibility to prime CD4+ T cells specific for conserved epitopes of a HIV protein is particularly important in view of the lack of such cells in HIV-infected individuals and of a possible role that CD4+ T cells may play in the development of protective immunity against AIDS.