EPR evidence for nitric oxide production from guanidino nitrogens of L-arginine in animal tissues in vivo.
EPR evidence for nitric oxide production from guanidino nitrogens of L-arginine in animal tissues in vivo.
复制标题
动物组织体内 L-精氨酸的胍基氮产生一氧化氮的 EPR 证据。
DOI：
10.1016/0005-2728(92)90032-w
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
A. Vanin
中科院分区：
文献类型：
--
作者：
L. Kubrina;W. Caldwell;P. Mordvintcev;I. V. Malenkova;A. Vanin
小鼠大腿皮下注射Fe 2+-柠檬酸盐络合物（FeSO 4或FeCl 2+柠檬酸钠）或腹腔注射大肠杆菌脂多糖（LPS）（1 mg/kg），均能诱导小鼠体内肝脏NO生成，其生成量为0.2-0.3 μg/g湿组织/0.5h。用内源性铁与二乙基二硫代氨基甲酸盐（DETC）形成的Fe 2+-二乙基二硫代氨基甲酸盐（FeDETC 2）特异性地捕获NO。NO与该陷阱结合导致形成顺磁性单亚硝基铁与DETC（NO-FeDETC 2）的配合物，其特征在于在g = 2.035，g= 2.02处具有EPR信号，在g = 2.03处具有三重态超精细结构（HFS）。这允许定量肝脏中形成的NO的量。一氧化氮合成酶抑制剂N-硝基-L-精氨酸（NNLA，50 mg/kg）可抑制NO的合成。l-精氨酸（500 mg/kg）逆转了这种作用。L-[胍基亚氨基-15N2]精氨酸与Fe 2+-柠檬酸盐或LPS联合注射可导致NO-FeDETC 2的EPR信号形成，其特征为在g/ml处的二重峰HFS，表明NO在体内来源于L-精氨酸的胍基氮。
Administration of Fe 2+-citrite complex (50 mg/kg of FeSO 4 or FeCl 2 plus 250 mg/kg of sodium citrate) subcutaneously in the thigh or Escherichia coli lipopolysaccharide (LPS, 1 mg/kg) intraperitoneally,(ip) to mice induced NO formation in the livers in vivo at the rate of 0.2–0.3 μg/g wet tissue per 0.5 h. The NO synthesized was specifically trapped with Fe 2+-diethyldithio-carbamate complex (FeDETC 2), tormed from endogenous iron and diethyldithiocarbamate (DETC) administered ip 0.5 h before decapitation of the animals. NO bound with this trap resulted in the formation of a paramagnetic mononitrosyl iron complex with DETC (NO-FeDETC 2), characterized by an EPR signal at g⊥= 2.035, g= 2.02 with triplet hyperfine structure (HFS) at g⊥. This allowed quantification of the amount of NO formed in the livers. An inhibitor of enzymatic NO synthesis from l-arginine, N G-nitro-l-arginine (NNLA, 50 mg/kg) attenuated the NO synthesis in vivo. l-Arginine (500 mg/kg) reversed this effect. Injection of l-[guanidineimino-15 N 2] arginine combined with Fe 2+-citrate or LPS led to the formation of the EPR signal of NO-FeDETC 2 characterized by a doublet HFS at g⊥, demonstrating that the NO originates from the guanidino nitrogens of l-arginine in vivo.
影响因子：
2.9
作者：
MARLETTA, MA;YOON, PS;WISHNOK, JS
通讯作者：
WISHNOK, JS