Propagation of an infertile hermaphrodite mouse lacking germ cells by using nuclear transfer and embryonic stem cell technology.
Propagation of an infertile hermaphrodite mouse lacking germ cells by using nuclear transfer and embryonic stem cell technology.
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DOI：
10.1073/pnas.0408548102
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发表时间：
2005-01
影响因子：
11.1
通讯作者：
中科院分区：
文献类型：
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通过系统诱变和常规育种产生的动物往往是不育的，因为它们缺乏生殖细胞，而维持这样的动物品系是不可能的。我们在我们的群体中发现了一只雌雄同体的不育小鼠，这是一只遗传雄性，Y染色体异常，缺乏发育中的生殖细胞。我们试图通过常规的核移植克隆这只老鼠，但没有成功。利用该小鼠的体细胞核移植（ntES细胞）克隆的囊胚产生的胚胎干细胞，注射到去核卵母细胞中也不能产生后代。虽然我们能够利用这些ntES细胞通过四倍体互补产生两个嵌合后代，但它们是不育的，因为它们也缺乏发育中的生殖细胞。然而，当这些ntES细胞被注射到正常的二倍体囊胚中时，产生了许多嵌合后代。其中一个这样的雄性后代通过携带X染色体的精子将雌雄同体的老鼠基因传递给有生育能力的后代。因此，ntES细胞被用于从缺乏生殖细胞的不育小鼠繁殖后代。
Animals generated by systematic mutagenesis and routine breeding are often infertile because they lack germ cells, and maintenance of such lines of animals has been impossible. We found a hermaphrodite infertile mouse in our colony, a genetic male with an abnormal Y chromosome lacking developing germ cells. We tried to clone this mouse by conventional nuclear transfer but without success. ES cells produced from blastocysts, which had been cloned by using somatic cell nuclear transfer (ntES cells) from this mouse, were also unable to produce offspring when injected into enucleated oocytes. Although we were able to produce two chimeric offspring using these ntES cells by tetraploid complementation, they were infertile, because they also lacked developing germ cells. However, when such ntES cells were injected into normal diploid blastocysts, many chimeric offspring were produced. One such male offspring transmitted hermaphrodite mouse genes to fertile daughters via X chromosome-bearing sperm. Thus, ntES cells were used to propagate offspring from infertile mice lacking germ cells.