PLT‐associated immunoglobulin increases during PLT storage
PLT‐associated immunoglobulin increases during PLT storage
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PLT 储存期间 PLT 相关免疫球蛋白增加
DOI：
10.1046/j.1537-2995.2003.00535.x
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发表时间：
2003
期刊：
影响因子：
2.9
通讯作者：
T. Koerner
中科院分区：
文献类型：
--
作者：
L. Cooling;T. Koerner
预防输血后 CMV 感染的策略包括检测 CMV 抗体、CMV DNA 和/或血液成分的 WBC 减少。 1, 2 早期的分子方法研究产生了相互矛盾的结果。然而，最近的研究表明，改进的 CMV PCR 检测不一定会增加健康 CMV 血清阴性献血者中 CMV DNA 的检测。 3, 4 我们在对来自奥地利维也纳地区 420 名捐献者的 244 份血浆和白细胞成分以及 176 份血清样本的研究中证实了这些发现（其中 384 份是随机选择的，36 份是针对 IgM 抗 CMV 反应性选择的样本）。我们使用两种 ELISA（Enzygnost 抗 CMV IgM 和 Enzygnost 抗 CMV IgG，Dade Behring，马尔堡，德国）测试了血清或血浆中的 IgM 和 IgG CMV 抗体。如果校正的吸光度值（DA=[A抗原-A对照抗原]¥校正因子）大于0.2 OD的截止值，则结果解释为阳性；如果吸光度值小于0.1 OD的截止值，则结果为阴性；如果第一次测试和重新测试中的吸光度值在0.1和0.2 OD之间，则结果不明确。使用 5 美分核酸酶实时 PCR 检测对血清、血浆和白细胞样本进行 CMV PCR 筛查，该检测针对病毒的聚合酶基因区域。 PCR 反应样品经过第二次实时检测重新测试并确认，该检测放大了主要立即早期区域的序列延伸。在定量血浆样品 VQC 1999/6 的连续稀释中，筛选测定的 95% 截止值估计为每个 PCR 四个拷贝（192 拷贝/mL）。在一名捐献者的白细胞分数和另一名捐献者的血清样本中可重复地检测和证实了 CMV DNA。在与 CMV DNA 阳性白细胞相对应的血浆或全血中未检测到 CMV DNA。我们对这些结果的解释表明该样本很可能来自潜伏感染者。这些样本中的病毒载量（约 100 拷贝/mL）比检测的阳性对照（1000 拷贝/mL）低约 10 倍。上述发现与 Roback 及其同事的研究结果非常吻合。 4 在他们的研究中，在 1000 个全血样本中有 2 个样本中每 250,000 个白细胞可检测到 10 至 99 个 CMV geq。两个样品均具有抗 CMV 反应性，但 IgM 和 IgG 反应性之间没有差异。我们还发现，DNA 阳性结果仅出现在血清阳性样本中，严格意义上仅限于 IgG 阳性以及 IgM 不确定或 IgM 阴性血清反应性（表 1）。
Strategies for preventing posttransfusion CMV infections include testing for CMV antibodies, CMV DNA, and/or WBC reduction of blood components. 1, 2 Early studies with molecular methods produced conflicting results. Nevertheless, recent investigations show that improved CMV PCR assays do not necessarily increase detection of CMV DNA in healthy CMV-seronegative blood donors. 3, 4 We confirm these findings in a study of 244 plasma and WBC fractions and 176 serum samples from 420 donors in the Vienna, Austria, region (384 randomly selected and 36 samples selected for IgM anti-CMV reactivity). We tested for IgM and IgG CMV antibodies in serum or plasma with two ELISAs (Enzygnost anti-CMV IgM and Enzygnost anti-CMV IgG, Dade Behring, Marburg, Germany). Results were interpreted to be positive if the corrected absorbance values (DA=[Aantigen–Acontrol antigen]¥ correction factor) were greater than the cutoff of 0.2 OD, negative if the absorbance values were less than the cutoff of 0.1 OD, and equivocal if the absorbance values were between 0.1 and 0.2 OD in the first test and in retesting. CMV PCR screening was performed on serum, plasma, and WBC samples with a 5¢-nuclease real-time PCR assay, which targets the polymerase gene region of the virus. PCR-reactive samples were retested and confirmed with a second real-time assay amplifying a sequence stretch in the major immediate early region. In serial dilutions of the quantitated plasma sample VQC 1999/6, the 95 percent cutoff value of the screening assay had been estimated to be four copies per PCR (192 copies/mL). CMV DNA was reproducibly detected and confirmed in the WBC fraction from one donor and a serum sample from another donor. CMV DNA was not detected in the plasma or whole blood corresponding to the CMV DNA-positive WBCs. We interpret these results to indicate that this sample was most probably from a latently infected individual. The viral loads in these samples were approximately 10 times lower (approx., 100 copies/mL) than the positive control for the assay (1000 copies/mL). The above findings correlate well with those of Roback and colleagues. 4 In their study, 10 to 99 CMV geq per 250,000 WBCs were detectable in 2 of 1000 whole blood samples. Both samples were reactive for anti-CMV but there was no differentiation between IgM and IgG reactivity. We also found our positive DNA results only in seropositive samples, in the strict sense restricted to IgG-positive and either IgM-equivocal or IgM-negative seroreactivity (Table 1).
唾液酸糖缀合物在红细胞衰老和隔离循环中的作用。
DOI：
--
发表时间：
1988
期刊：
Blood cells
影响因子：
--
作者：
Aminoff,D
通讯作者：
Aminoff,D