Basal cell subpopulation as putative human prostate carcinoma stem cells.
Basal cell subpopulation as putative human prostate carcinoma stem cells.
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DOI：
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发表时间：
2007
影响因子：
1.5
通讯作者：
A. Kuzmanov;S. Hayrabedyan;M. Karaivanov;K. Todorova
中科院分区：
文献类型：
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作者：
A. Kuzmanov;S. Hayrabedyan;M. Karaivanov;K. Todorova
本研究检测了增生性炎性萎缩（PIA）组织中p63、谷胱甘肽S-转移酶-π（GSTP 1）和α-甲酰基辅酶AMACR（AMACR）的表达，以探讨某些基底细胞可能是假定的人前列腺癌干细胞（PHPCSC）。使用抗AMACR（Dako）、p63和GSTP 1（Labvision）的抗体的免疫组织化学测试在根治性直肠切除术后从包含PIA的病例（n=30）获得的存档组织切片，并通过生物素-链霉亲和素-过氧化物酶试剂盒（DAKO LSAB 2试剂盒）可视化。定量免疫组织化学分析（QIHC）的研究抗原表达水平显示，有两个群体的p63基底细胞。Ⅰ型基底细胞AMACR高表达，GSTP 1和p63低表达。Ⅱ型基底细胞AMACR低，GSTP 1和p63高表达。因此，我们提出，推定的人前列腺癌干细胞可能居住在人口的I型基底细胞。
The present study examines the expression of p63, glutathione S-transferase-pi (GSTP1) and alpha-methylacyl-CoAracemase (AMACR) in serial slices in proliferative inflammatory atrophy (PIA) in order to implicate that some of the basal cells are probably the putative human prostate carcinoma stem cells (PHPCSC). Archived tissue sections obtained after radical prostatectomy from cases (n=30) comprising of PIA were tested using immunohistochemistry with antibodies against AMACR (Dako), p63 and GSTP1 (Labvision) and visualized by biotin-streptavidin-peroxidase kit (DAKO LSAB 2 kit). Quantitative immunohistochemistry analysis (QIHC) of the studied antigen expression levels revealed that there are two populations of p63 basal cells. Type I basal cells had high AMACR, low GSTP1 and p63 expression. Type II basal cells had low AMACR, high GSTP1 and p63 expression. Therefore, we propose that the putative human prostate carcinoma stem cells probably reside within the population of type I basal cells.