Similar level of metabolic activation of benzo(a)pyrene in perfused rat lung and liver and protection of lung by liver in a combined perfusion system.
Similar level of metabolic activation of benzo(a)pyrene in perfused rat lung and liver and protection of lung by liver in a combined perfusion system.
复制标题
灌注大鼠肺和肝脏中苯并(a)芘的代谢激活水平相似，并且在组合灌注系统中肝脏对肺的保护作用相似。
DOI：
10.1016/0006-291x(82)91476-0
复制
发表时间：
1982
影响因子：
3.1
通讯作者：
G. F. Kahl
中科院分区：
文献类型：
--
作者：
E. Klaus;H. Foth;R. Kahl;G. F. Kahl
代谢活化的苯并（a）芘与DNA，RNA和蛋白质的不可逆结合在5，6-benzodironone处理的大鼠的灌注肝和肺中通过不同的时间过程进行。在肝脏中的峰值结合后，15分钟，而在肺中的结合持续增加超过120分钟。总的不可逆结合每毫克DNA或RNA是在两个器官的数量级相同。虽然在肺中的结合是较低的15分钟，它超过了在120分钟在肝脏中的结合。结合每毫克蛋白是在肺高于在肝脏在整个灌注期间。将肝脏引入肺灌注回路减少了肺中的结合。当肝脏是从用5，6-苯丙酮预处理的动物中获得时，这种保护作用更加明显。
Irreversible binding of metabolically activated benzo(a)pyrene to DNA, RNA and protein proceeds by a different time course in perfused liver and lung of 5,6-benzoflavone-treated rats. Peak binding in liver is obtained after 15 min while binding in lung continuously increases over 120 min. Total irreversible binding per mg DNA or RNA is in the same order of magnitude in both organs. While binding in lung is lower at 15 min it exceeds binding in liver at 120 min. Binding per mg protein is higher in lung than in liver over the whole perfusion period. Introduction of a liver into the lung perfusion circuit decreases binding in lung. This protection effect is more pronounced when the liver is obtained from an animal pretreated with 5,6-benzoflavone.