Establishment of cell model of Radiation-induced lung injury
Establishment of cell model of Radiation-induced lung injury
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放射性肺损伤细胞模型的建立
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通讯作者：
侯炜
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作者：
王家伟;王雪凯;隋宇;张解玉;侯炜
目的：探讨大鼠肺泡巨噬细胞NR8383体外模型的建立及鉴定方法：采用医用电子直线加速器，分别以0Gy和6GyX系照射细胞。在6h、12h、24h、30h、48h五个时间点后，观察两组小鼠DNA损伤指数（8-OHdG）、脂质损伤指数（MDA），以及TNF-α、TGF-β、IL-6的含量。结果:1。6Gy照射组8-OHdG和MDA含量在照射后不同时间点均高于正常组，在照射后约6h达到最高值，之后逐渐降低，趋于平缓。2.6Gy照射组在照射后不同时间点炎症因子TNF-α、TGF-β、IL-6水平均高于正常组。结论：6Gy X射线照射大鼠肺泡巨噬细胞（NR8383）可建立体外放射性肺损伤细胞模型。DNA损伤指数（8-OHdG）、脂质损伤指数（MDA）、TNF-α、TGF-β、IL-6均可作为有效的评价指标。
Objective: To explore the establishment and identification of rat lung alveolar macrophage NR8383 model in vitro..Methods: using the medical electronic linear accelerator, the cells were irradiated with 0Gy and 6GyX lines respectively. After the five time points of 6h, 12h, 24h, 30h, 48h, the DNA damage index (8-OHdG) and lipid damage index (MDA) were observed in two groups, as well as the content of TNF-α , TGF-β and IL-6..Results: 1.The contents of 8-OHdG and MDA in 6Gy irradiation group were higher than those in the normal group at different time points after irradiation, and reached the highest value at about 6h after irradiation, and then gradually decreased and tended to be gentle. 2.The levels of inflammatory factors TNF-α , TGF-β and IL-6 in 6Gy irradiation group were higher than those in normal group at different time points after irradiation.Conclusion：6Gy X ray irradiated rat alveolar macrophages(NR8383) can establish an in-vitro cell model radiation lung injury. DNA damage index (8-OHdG), lipid damage index (MDA), TNF-α , TGF-β and IL-6 could be used as effective evaluation indicators.