Quantitative and qualitative analysis of Botrytis inoculated on table grapes by qPCR and antibodies
Quantitative and qualitative analysis of Botrytis inoculated on table grapes by qPCR and antibodies
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DOI：
10.1016/j.postharvbio.2008.10.007
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发表时间：
2009-05-01
影响因子：
7
通讯作者：
Lichter, Amnon
中科院分区：
文献类型：
--
作者：
Celik, Mustafa;Kalpulov, Tatiana;Lichter, Amnon
葡萄灰霉病菌是鲜食葡萄储存过程中腐烂的主要原因，腐烂的严重程度部分取决于储存前真菌的污染。目前防止腐烂的SO2技术是可靠的，并且与灰孢杆菌的污染水平无关。然而，采用替代技术可能需要采用与污染程度成比例的手段。本研究的目的是检验在人工接种葡萄中定量葡萄灰孢杆菌的可行性，并监测贮藏过程中病害的进展。比较了两种检测葡萄灰霉病菌的方法：葡萄灰霉病菌特异性抗体试剂盒和真菌特异性引物定量PCR。快速检测灰绿杆菌的抗体仅在腐烂发展的后期产生阳性结果。相比之下，定量PCR在所有储存时间点都显示出真菌的阳性鉴定，并且在储存期间发现真菌的数量增加。(C) 2008 Elsevier B.V.版权所有
The fungus Botrytis cinerea is the major cause of decay in table grapes during storage, and the severity of decay depends in part on contamination with the fungus before storage. The current SO2 technology to prevent decay is robust and independent of the level of contamination by B. cinerea. The introduction of alternative technologies may however require implementation of means which are proportional to the level of contamination. The objectives of this study were to test the feasibility of quantifying B. cinerea in artificially inoculated grapes and to monitor the progress of disease during storage. Two methods were compared for detection of B. cinerea in grapes: an antibody kit specific for B. cinerea, and quantitative PCR using fungal specific primers. Antibodies for fast detection of B. cinerea yielded positive results only in the later stages of decay development. In contrast, the quantitative PCR demonstrated positive identification of the fungus at all storage time points, and found increasing amounts of the fungus during storage. (C) 2008 Elsevier B.V. All rights reserved.