Structure of neurohypophysial hormone genes and changes in the levels of expression during spawning season in grass puffer (Takifugu niphobles)
Structure of neurohypophysial hormone genes and changes in the levels of expression during spawning season in grass puffer (Takifugu niphobles)
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DOI：
10.1016/j.ygcen.2007.07.009
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发表时间：
2008-01-15
影响因子：
2.7
通讯作者：
Ando, Hironorl
中科院分区：
文献类型：
--
作者：
Mototiashi, Eiji;Hamabata, Tomoko;Ando, Hironorl
Vasotocin（VT）已被证明影响广泛的脊椎动物物种的社会和性行为的各个方面，但对这种肽调节行为的机制知之甚少。此外，对异催产素（IT）在行为调节中的作用知之甚少。草河豚（Takifugu niphobles）具有独特的产卵行为，仅在大潮前后的几天内在海滩上产卵，由10-60只个体组成群体，其中1只为雌性。作为研究VT和IT在该物种产卵行为中作用的第一步，我们确定了草河豚VT和IT基因的结构。近缘种虎河豚和绿色河豚的基因组资源。然后，我们使用这些序列开发实时PCR检测和检查的VT和IT基因的表达在产卵季节的变化。VT和IT基因的结构在三种河豚鱼中具有很好的保守性。特别是，草河豚和虎河豚之间的序列相似性非常高，不仅在编码区（85-99%），而且在非编码区（92-98%），包括5 '-上游区域。VT基因在产卵前雌鱼脑中的表达量增加。产卵雌鱼VT mRNA的表达水平有高于产卵雄鱼的趋势。相比之下，IT mRNA的水平没有显示出这种变化。本研究结果表明，VT基因的表达增强，在产卵期的女性的大脑。草河豚独特的产卵行为为利用虎河豚基因组资源研究性行为的分子机制提供了有用的模型。(c)2007年爱思唯尔公司All rights reserved.
Vasotocin (VT) has been shown to influence various aspects of social and sexual behaviors in a broad range of vertebrate species, but less is known about the mechanisms through which this peptide modulates behavior. Additionally, much less is known about roles of isotocin (IT) in regulation of behavior. Grass puffer, Takifugu niphobles, has unique spawning behavior; spawning occurs on beach only for several days around the spring tide and is conducted by a group of 10-60 individuals, of which one is female. As a first step toward investigating the roles of VT and IT in this species' spawning behavior, we determined the structures of the VT and IT genes from grass puffer using! the genome resources of the closely related tiger puffer and green puffer. We then used these sequences to develop real-time PCR assays and examined changes in expression of the VT and IT genes over the spawning season. The structures of VT and IT genes are well conserved among three puffer species. Particularly, the sequence similarities between grass and tiger puffers were very high not only in the coding region (85-99%), but also in the non-coding regions (92-98%) that include the 5'-upstream regions. The levels of expression of VT gene increased in the brain of pre-spawning females. The levels of VT mRNA in the spawning females tended to be higher than that in the spawning males. In contrast, the levels of IT mRNA did not show such variation. The present results suggest that VT gene expression augments in the brain of females during the spawning period. The unique spawning behavior of grass puffer provides a useful model for studying the molecular mechanism of sexual behavior utilizing the genome resources of tiger puffer. (c) 2007 Elsevier Inc. All rights reserved.