Molecular characterization and functional distribution of N-ethylmaleimide-sensitive factor in Helicoverpa armigera
Molecular characterization and functional distribution of N-ethylmaleimide-sensitive factor in Helicoverpa armigera
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DOI：
10.1016/j.peptides.2005.11.011
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发表时间：
2006-06-01
期刊：
影响因子：
3
通讯作者：
Xu, Wei-Hua
中科院分区：
文献类型：
--
作者：
Cui, Shuai-Ying;Xu, Wei-Hua
N-乙基马来酰亚胺敏感性融合蛋白（NSF）是存在于所有真核细胞中的神经递质或神经激素释放装置的必需组分。在这里，一个新的NSF直向同源物的特点是从棉铃虫，棉铃虫（Har）。北方印迹显示在幼虫脑中有高表达。Southern分析表明，该基因的单拷贝存在于单倍体基因组中。利用荧光素标记的抗体，我们直接证明了NSF与两种重要的神经激素，促前胸腺激素和滞育激素，这两种激素都调节昆虫的发育。这些结果表明，Har-NSF可能参与调节昆虫神经激素的释放。(c)2005年爱思唯尔公司All rights reserved.
N-ethylmaleimide-sensitive fusion protein (NSF) is an essential component for the neurotransmitter or neurohormone release apparatus present in all eukaryotic cells. Here, a new NSF orthologue was characterized from the cotton bollworm, Helicoverpa armigera (Har). Northern blot exhibited a high expression in larval brain. Southern analysis indicated that a single copy of the gene is present in a haploid genome. Using antibodies labeled with fluoresceins, we directly proved that NSF is co-localized with two crucial neurohormones, prothoracicotropic hormone and diapause hormone, both of which regulate insect development. These findings suggest that Har-NSF may be involved in regulating insect neurohormone release. (c) 2005 Elsevier Inc. All rights reserved.