Mechanisms associated with cellular desiccation tolerance of Artemia encysted embryos from locations around the world
Mechanisms associated with cellular desiccation tolerance of Artemia encysted embryos from locations around the world
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DOI：
10.1016/j.cbpa.2011.05.032
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发表时间：
2011-10-01
影响因子：
2.3
通讯作者：
Clegg, J. S.
中科院分区：
文献类型：
--
作者：
Hengherr, Steffen;Schill, Ralph O.;Clegg, J. S.
用差示扫描量热法测定了11个不同地点卤虫干包囊原肠胚(囊)的玻璃化转变温度(Tg)，其中2个囊囊来自孤雌生殖动物。到目前为止，来自越南湄公河三角洲(VN)的贫血Franciscana包囊的TG值最高，这些包囊是从美国加利福尼亚州旧金山湾的包囊生长池中连续接种产生的。三组法兰西沙门氏菌的Tg值显著高于所研究的其他包囊(除变态卤虫外)，以及迄今为止所研究的所有其他耐脱水动物系统。我们还检测了所有这些包囊中的三种应激蛋白(hsc70、Artemin和P26)以及总的醇溶碳水化合物(ASC)，其中约90%是生物玻璃的已知成分--二糖海藻糖。我们根据与脱水耐受性有关的机制以及在某种程度上与囊肿收集部位的热条件相关的机制来解释结果(C)2011爱思唯尔公司。保留所有权利。
Using differential scanning calorimetry we demonstrated the presence of biological glasses and measured the glass transition temperatures (Tg) in dry encysted gastrula embryos (cysts) of the brine shrimp, Artemia, from eleven different locations, two of which provided cysts from parthenogenetic animals. Values for Tg were highest, by far, in Anemia franciscana cysts from the Mekong Delta, Vietnam (VN), these cysts having been produced from previous sequential inoculations into growth ponds of cysts from the San Francisco Bay, California, USA. Tg values for three groups of A. franciscana cysts were significantly higher than those of other cysts (except those of Artemia persimilis) studied here, as well as all other desiccation-tolerant animal systems studied to date. We also measured three stress proteins (hsc70, artemin and p26) in all these cysts as well as the total alcohol soluble carbohydrates (ASC), about 90% of which is the disaccharide trehalose, a known component of biological glasses. We interpret the results in terms of mechanisms involved with desiccation tolerance and, to some extent, with thermal conditions at the sites of cyst collection (C) 2011 Elsevier Inc. All rights reserved.