DC-SIGNR, a DC-SIGN homologue expressed in endothelial cells, binds to human and simian immunodeficiency viruses and activates infection in trans
DC-SIGNR, a DC-SIGN homologue expressed in endothelial cells, binds to human and simian immunodeficiency viruses and activates infection in trans
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DOI：
10.1073/pnas.051631398
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发表时间：
2001-02-27
影响因子：
11.1
通讯作者：
Doms, RW
中科院分区：
文献类型：
--
作者：
Pöhlmann, S;Soilleux, EJ;Doms, RW
DC-SIGN是一种表达在树突状细胞（dc）表面的c型凝集素，它能有效地结合hiv和猿猴免疫缺陷病毒并将其传播到易感细胞上，最近有研究报道了DC-SIGN的一种同系物，称为DC-SIGNR。本研究表明，DC-SIGNR和DC-SIGN一样，可以结合多种HIV-1、HIV-2和猴免疫缺陷病毒株，并将这些病毒传播到T细胞系和人外周血单核细胞。病毒与DC-SIGNR的结合依赖于碳水化合物的识别。DC-SIGNR特异性抗血清免疫染色显示DC-SIGNR在肝窦内皮细胞、淋巴结窦内皮细胞和胎盘绒毛上表达。这种有效的病毒附着因子在多种内皮细胞类型上的存在表明DC-SIGNR可能在灵长类慢病毒的垂直传播中发挥作用，使HIV能够穿过某些器官的毛细血管内皮，并在包括淋巴结在内的多个位置向cd4阳性细胞呈递病毒。
DC-SIGN, a C-type lectin expressed on the surface of dendritic cells (DCs), efficiently binds and transmits HIVs and simian immunodeficiency viruses to susceptible cells in trans, A DC-SIGN homologue, termed DC-SIGNR, has recently been described. Herein we show that DC-SIGNR, like DC-SIGN, can bind to multiple strains of HIV-1, HIV-2, and simian immunodeficiency virus and transmit these viruses to both T cell lines and human peripheral blood mononuclear cells. Binding of virus to DC-SIGNR was dependent on carbohydrate recognition. Immunostaining with a DC-SIGNR-specific antiserum showed that DC-SIGNR was expressed on sinusoidal endothelial cells in the liver and on endothelial cells in lymph node sinuses and placental villi. The presence of this efficient virus attachment factor on multiple endothelial cell types indicates that DC-SIGNR could play a role in the vertical transmission of primate lentiviruses, in the enabling of HIV to traverse the capillary endothelium in some organs, and in the presentation of virus to CD4-positive cells in multiple locations including lymph nodes.