SIMULTANEOUS MEASUREMENTS OF UMBILICAL UPTAKE, FETAL UTILIZATION RATE, AND FETAL TURNOVER RATE OF GLUCOSE
SIMULTANEOUS MEASUREMENTS OF UMBILICAL UPTAKE, FETAL UTILIZATION RATE, AND FETAL TURNOVER RATE OF GLUCOSE
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DOI：
10.1152/ajpendo.1981.240.6.e662
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发表时间：
1981-01-01
影响因子：
--
通讯作者：
MESCHIA, G
中科院分区：
文献类型：
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作者：
HAY, WW;SPARKS, JW;MESCHIA, G
在12只平均胎龄为137天（范围：118-146天）的妊娠绵羊中，将胎儿脐葡萄糖摄取与葡萄糖周转率和利用率的同时测量进行了比较。通过应用Fick原理计算葡萄糖摄取。通过[14 C]-和[3 H]-葡萄糖的预充-恒定输注测量胎儿葡萄糖周转率（葡萄糖周转率=示踪剂输注率胎儿葡萄糖比活度）。胎儿葡萄糖利用率的计算需要从示踪剂输注速率中减去胎盘示踪剂的损失，从而定义进入胎儿的示踪剂净量，以便与脐带葡萄糖净摄取量进行直接比较。在进食的血糖正常的绵羊中，这些测量结果表明脐葡萄糖吸收率（4.77 mg. cntdot.）具有统计学等效性。min-1. kg-1 ±. 0.34 SE）和葡萄糖利用率（[14 C]葡萄糖，5.58 mg. min-1. kg-1 ±. 0.54 SE;和[3 H]葡萄糖，7.19 mg. min-1. kg-1 ±. 1.24 SE），经双因素方差分析，P > 0.01。在3只禁食的低血糖绵羊中，脐葡萄糖摄取率降至1.43 mg. cntdot。min-1. kg-1 ±. 0.56 SE，其显著低于同时葡萄糖利用率（[14 C]葡萄糖，4.78 mg. min-1. kg-1 ±. 0.48 SE;和[3 H]葡萄糖，6.81 mg. min-1. kg-1 ±. 2.19 SE）。在血糖正常的妊娠晚期胎羊中，似乎几乎没有葡萄糖生成;在禁食诱导的胎儿低血糖期间，葡萄糖生成可能变得显著。
Fetal umbilical glucose uptake was compared with simultaneous measurements of glucose turnover and utilization rates in 12 pregnant sheep, at a mean of 137 days gestational age (range, 118-146 days). Umbilical glucose uptake was calculated by application of the Fick principle. Fetal glucose turnover rate was measured by a primed-constant infusion of [14C]- and [3H]glucose (glucose turnover rate = tracer infusion rate .div. fetal glucose specific activity). The calculation of fetal glucose utilization rate required substraction of the loss of tracer to the placenta from the tracer infusion rate, thus defining the net tracer entry into the fetus for direct comparison with the net umbilical glucose uptake. In fed, normoglycemic sheep, these measurements demonstrated statistical equivalence of umbilical glucose uptake rate (4.77 mg .cntdot. min-1 .cntdot. kg-1 .+-. 0.34 SE) and glucose utilization rate ([14C]glucose, 5.58 mg .cntdot. min-1 .cntdot. kg-1 .+-. 0.54 SE; and [3H]glucose, 7.19 mg .cntdot. min-1 .cntdot. kg-1 .+-. 1.24 SE) when tested by 2-way analysis of variance (P > 0.01). In 3 fasted, hypoglycemic sheep, the umbilical glucose uptake rate fell to 1.43 mg .cntdot. min-1 .cntdot. kg-1 .+-. 0.56 SE, which was considerably lower than the simultaneous glucose utilization rate ([14C]glucose, 4.78 mg .cntdot. min-1 .cntdot. kg-1 .+-. 0.48 SE; and [3H]glucose, 6.81 mg .cntdot. min-1 .cntdot. kg-1 .+-. 2.19 SE). In the normoglycemic, late-gestation fetal lamb, there appears to be little glucogenesis; glucogenesis may become significant during fasting-induced fetal hypoglycemia.