Anti-Quorum Sensing Activity of Forsythia suspense on Chromobacterium violaceum and Pseudomonas aeruginosa.
Anti-Quorum Sensing Activity of Forsythia suspense on Chromobacterium violaceum and Pseudomonas aeruginosa.
复制标题
DOI：
10.4103/0973-1296.204547
复制
发表时间：
2017-04
影响因子：
0.7
通讯作者：
Chu WH
中科院分区：
文献类型：
--
作者：
Zhang A;Chu WH
群体感应（Quorum sensing，QS）在铜绿假单胞菌毒力因子的产生和致病性中起着重要作用，阻断QS将是对抗细菌感染的一条有希望的途径。本研究选用连翘Forsythia suspension（Thunb.）Vahl来源于传统中草药，具有抗QS活性。抗F.使用紫色色杆菌12472生物测定监测悬液提取物（FSE）。使用标准方法研究FSE对铜绿假单胞菌PAO 1中QS控制的毒力因子产生、游泳运动性和生物膜建立的影响。FSE能明显抑制C. violaceum 12472对铜绿假单胞菌群体感应调节的毒力因子的产生和生物被膜的形成具有浓度依赖性抑制作用。0.25 g/mL的FSE对铜绿假单胞菌的弹性蛋白酶活性和绿脓菌素产量的抑制率分别为40.97%和47.58%，对铜绿假单胞菌的游泳运动也有抑制作用。当添加0.25 g/mL的FSE时，PAO 1的生物膜形成能力下降了72.45%。结果提示，FSE可作为一种基于QS抑制的替代药物来控制和处理细菌病原体引起的有害感染。连翘水提液对连翘紫色素的产生有明显的抑制作用。连翘水提取物能抑制铜绿假单胞菌QS调节的毒力因子的产生和生物被膜的形成。使用的缩略语：QS：群体感应，铜绿假单胞菌，连翘。悬念，FSE：F.悬液提取物，紫色色杆菌12472 C. violaceum 12472，AIs：自身诱导剂，AHL：N-酰基高丝氨酸内酯，LB：Luria-Bertani，MIC：最小抑制浓度，CFU：菌落形成单位，ATCC：美国典型培养物保藏中心，PBS：磷酸盐缓冲盐水
Quorum sensing (QS) plays an important role in the production of virulence factors and pathogenicity in Pseudomonas aeruginosa, and the interruption of QS will be a hopeful pathway to combat bacterial infection. In this study, we selected Forsythia suspense (Thunb.) Vahl from traditional Chinese herbal medicines for its anti-QS activity. Anti-QS of F. suspense extracts (FSE) was monitored using the Chromobacterium violaceum 12472 bioassay. Standard methods were used to investigate the effects of FSE on QS-controlled virulence factors production, swimming motility, and biofilm establishment in P. aeruginosa PAO1. FSE could obviously inhibit the violacein production in C. violaceum 12472 and also could inhibit quorum sensing–regulated virulence factors production and biofilm formation in P. aeruginosa in a concentration-dependent manner. The elastase activity and pyocyanin production were inhibited at a maximum of 40.97 and 47.58% when P. aeruginosa was grown in the presence of 0.25 g/mL FSE, which can also inhibit swimming motility of P. aeruginosa. The biofilm formation ability was decreased about 72.45% when in PAO1 cultured with the 0.25 g/mL FSE. The results suggested that FSE may be used as an alternative drug to control and handle harmful infections caused by bacterial pathogens based on QS inhibition. Forsythia suspense water extract could obviously inhibit the purple pigment production in C. violaceum 12472 Forsythia suspense water extract could inhibit QS-regulated virulence factors production and biofilm formation in P. aeruginosa. Abbreviations used: QS: Quorum sensing, Pseudomonas aeruginosa P. aeruginosa, Forsythia suspense F. suspense, FSE: F. suspense extracts, Chromobacterium violaceum 12472 C. violaceum 12472, AIs: autoinducers, AHLs: N-acyl-homoserinelactones, LB: Luria-Bertani, MICs: Minimum inhibitory concentrations, CFU: Colony-Forming Units, ATCC: American Type Culture Collection, PBS: phosphate buffered saline